Transglutaminase 2 antibody

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Goat anti Human Transglutaminase 2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2142 E WB 0.1 mg
Goat anti Human transglutaminase 2 antibody recognizes human Protein-glutamine gamma-glutamyltransferase 2, also known as Transglutaminase 2 (TGM2), or tissue Transglutaminase (tTG). TGM2 is a 687 amino acid member of the Transglutaminase family. It is a ubiquitously expressed calcium-dependent enzyme, with GTPase activity, which catalyzes protein cross-linking and the conjugation of polyamines to proteins.

TGM2 has been implicated in several processes, including apoptosis (Hseih et al. 2013), the regulation of cytoskeletal structure, cell-matrix adhesion, cell migration, and wound healing (Fesus and Piacentini 2002). Antibodies against tTG are apparent in a high majority of patients with the autoimmune condition known as celiac disease, resulting in sensitivity to gluten (Gelderman et al. 2014). Three isoforms of TGM2 generated by alternative splicing exist. Goat anti Human transglutaminase 2 antibody is generated against a peptide spanning amino acid residues 667-680 of isoform 1. It does not recognize the other isoforms having carboxy-terminal deletions.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human TGM2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-NFESDKLKAVKGFR from the internal region (near the C- Terminal) of TGM2 (NP_004604.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P21980 Related reagents
Entrez Gene
TGM2 Related reagents
GO Terms
GO:0003810 protein-glutamine gamma-glutamyltransferase activity
GO:0046872 metal ion binding
GO:0008415 acyltransferase activity
GO:0043277 apoptotic cell clearance
GO:0045785 positive regulation of cell adhesion
Regulatory
For research purposes only

Applications of Transglutaminase 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/128000
Western Blotting 0.03 0.1ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2142 detects a band of approximately 90kDa in human lung cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB