Transglutaminase 2 antibody
Goat anti Human transglutaminase 2 antibody recognizes human Protein-glutamine gamma-glutamyltransferase 2, also known as Transglutaminase 2 (TGM2), or tissue Transglutaminase (tTG). TGM2 is a 687 amino acid member of the Transglutaminase family. It is a ubiquitously expressed calcium-dependent enzyme, with GTPase activity, which catalyzes protein cross-linking and the conjugation of polyamines to proteins.
TGM2 has been implicated in several processes, including apoptosis (Hseih et al. 2013), the regulation of cytoskeletal structure, cell-matrix adhesion, cell migration, and wound healing (Fesus and Piacentini 2002). Antibodies against tTG are apparent in a high majority of patients with the autoimmune condition known as celiac disease, resulting in sensitivity to gluten (Gelderman et al. 2014). Three isoforms of TGM2 generated by alternative splicing exist. Goat anti Human transglutaminase 2 antibody is generated against a peptide spanning amino acid residues 667-680 of isoform 1. It does not recognize the other isoforms having carboxy-terminal deletions.
TGM2 has been implicated in several processes, including apoptosis (Hseih et al. 2013), the regulation of cytoskeletal structure, cell-matrix adhesion, cell migration, and wound healing (Fesus and Piacentini 2002). Antibodies against tTG are apparent in a high majority of patients with the autoimmune condition known as celiac disease, resulting in sensitivity to gluten (Gelderman et al. 2014). Three isoforms of TGM2 generated by alternative splicing exist. Goat anti Human transglutaminase 2 antibody is generated against a peptide spanning amino acid residues 667-680 of isoform 1. It does not recognize the other isoforms having carboxy-terminal deletions.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human TGM2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence C-NFESDKLKAVKGFR from the internal region (near the C- Terminal) of TGM2 (NP_004604.2).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21980
- Entrez Gene
- TGM2
- GO Terms
- GO:0003810 protein-glutamine gamma-glutamyltransferase activity
- GO:0046872 metal ion binding
- GO:0008415 acyltransferase activity
- GO:0043277 apoptotic cell clearance
- GO:0045785 positive regulation of cell adhesion
- Regulatory
- For research purposes only
Applications of Transglutaminase 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/128000 | ||
| Western Blotting | 0.03 | 0.1ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP2142 detects a band of approximately 90kDa in human lung cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
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