Goat anti Human transglutaminase 2 antibody
recognizes human Protein-glutamine gamma-glutamyltransferase 2, also known as Transglutaminase 2 (TGM2), or tissue Transglutaminase (tTG). TGM2 is a 687 amino acid member of the Transglutaminase family. It is a ubiquitously expressed calcium-dependent enzyme, with GTPase activity, which catalyzes protein cross-linking and the conjugation of polyamines to proteins.
TGM2 has been implicated in several processes, including apoptosis (Hseih et al. 2013
), the regulation of cytoskeletal structure, cell-matrix adhesion, cell migration, and wound healing (Fesus and Piacentini 2002
). Antibodies against tTG are apparent in a high majority of patients with the autoimmune condition known as celiac disease, resulting in sensitivity to gluten (Gelderman et al. 2014
). Three isoforms of TGM2 generated by alternative splicing exist. Goat anti Human transglutaminase 2 antibody is generated against a peptide spanning amino acid residues 667-680 of isoform 1. It does not recognize the other isoforms having carboxy-terminal deletions.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Transglutaminase 2 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP2142 detects a band of approximately 90kDa in human lung cell lysates.