Transferrin antibody

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Anti Transferrin Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti Transferrin Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti Transferrin Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti Transferrin Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti Human transferrin antibody (**VPA00797**) followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to transferrin (molecular weight 77 kDa).

Anti Transferrin Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HepG2 separately transfected with two unique siRNA duplexes against Transferrin (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti Transferrin antibody (**VPA00797**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 30 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti Transferrin Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of Transferrin from whole cell lysates prepared from HepG2 separately transfected with two unique siRNA duplexes against Transferrin (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Rabbit anti Transferrin

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Transferrin

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VPA00797 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human transferrin antibody recognizes transferrin, also known as serotransferrin, beta-1 metal-binding globulin or siderophilin.

TF encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum (provided by RefSeq, Sep 2009).

Rabbit anti Human transferrin antibody detects a band of 77 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti transferrin antibody recognizes a band of approximately 77 kDa in HepG2 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Oryza sativa-derived recombinant protein of amino acids 20-698 of human transferrin
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P02787
Entrez Gene: TF
GO Terms: GO:0002576 platelet degranulation; GO:0005576 extracellular region; GO:0005769 early endosome; GO:0005770 late endosome; GO:0030141 stored secretory granule; GO:0030139 endocytic vesicle; GO:0005905 coated pit; GO:0006879 cellular iron ion homeostasis; GO:0008199 ferric iron binding; View More GO Terms; GO:0009925 basal plasma membrane; GO:0010008 endosome membrane; GO:0016324 apical plasma membrane; GO:0030168 platelet activation; GO:0033572 transferrin transport; GO:0048471 perinuclear region of cytoplasm; GO:0055037 recycling endosome; GO:0055085 transmembrane transport