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Syk antibody

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Western blotting analysis of Human spleen lysate (35μg protein in RIPA buffer) using Goat anti Human SYK antibody (AHP1066) at 1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

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WB

Goat anti Syk

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Syk

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Product Code Applications Pack Size List Price Your Price Qty
AHP1066
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg
List Price Your Price

Goat anti Syk antibody detects an epitope within the internal region of SYK, a ~70 kDa non-receptor protein tyrosine kinase whose role differs in haematopoietic and non haematopoietic stem cells. In haematopoietic cells SYK is involved in the proximal signalling downstream of activated immunoreceptors (BCR, TCR, Fc receptors), thereby orchestrating a complex series of cellular responses such as cell proliferation, differentiation, survival and phagocytosis.

In epithelial cells SYK appears to function as a negative regulator of related events. Furthermore, SYK has been shown to promote expression or secretion of inflammation-related molecules in lung epithelial cells.

SYK appears to be required for vascular integrity during development, thus, manipulation of SYK function in endothelial cells could potentially modulate tumour-associated angiogenesis. SYK also has been shown to interact with Epstein-Barr virus LMP2A.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Dog Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to SYK were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence C-KYLQQNRHVKDKN from the internal region of the SYK sequence.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16000
Western Blotting 1.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1066 detects a band of approximately 70kDa in human bone marrow, lymph node and spleen lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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Description Rabbit anti Goat IgG (Fc):HRP

References for Syk antibody

  1. Coopman, P.J. & Mueller, S.C. (2006) The Syk tyrosine kinase: a new negative regulator in tumor growth and progression.
    Cancer Lett. 241 (2): 159-73.
  2. Wong, B.R. et al. (2004) Targeting Syk as a treatment for allergic and autoimmune disorders.
    Expert Opin Investig Drugs. 13 (7): 743-62.

Synonyms
Spleen Tyrosine Kinase
RRID
AB_2200254
UniProt
P43405
Entrez Gene
SYK
GO Terms
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005178 integrin binding
GO:0005524 ATP binding
GO:0006461 protein complex assembly
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0009887 organ morphogenesis
GO:0008283 cell proliferation
GO:0030168 platelet activation
GO:0030593 neutrophil chemotaxis
GO:0042101 T cell receptor complex
GO:0044419 interspecies interaction between organisms

AHP1066

157057 158641 210114

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