Superoxide Dismutase (MN) antibody

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Rabbit anti Superoxide Dismutase (MN)

Superoxide dismutase (Mn) antibody recognizes superoxide dismutase (Mn), also known as superoxide dismutase 2, SOD2, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase or mangano-superoxide dismutase.

Superoxide Dismutase (MN) Antibody with Control Lysate

Superoxide dismutase (Mn) antibody recognizes superoxide dismutase (Mn), also known as superoxide dismutase 2, SOD2, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase or mangano-superoxide dismutase.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00802 WB 100 µl
VPA00802KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human superoxide dismutase (Mn) recognizes superoxide dismutase (Mn), also known as superoxide dismutase 2, SOD2, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase or mangano-superoxide dismutase.

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1 (provided by RefSeq, Apr 2016).

Rabbit anti Human superoxide dismutase (Mn) detects a band at 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti superoxide dismutase (Mn) antibody recognizes a band of approximately 22 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human superoxide dismutase (Mn)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P04179 Related reagents
Entrez Gene
SOD2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0001315 age-dependent response to reactive oxygen species
GO:0001836 release of cytochrome c from mitochondria
GO:0003069 vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure
GO:0004784 superoxide dismutase activity
GO:0005759 mitochondrial matrix
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0019430 removal of superoxide radicals
GO:0030145 manganese ion binding
GO:0032364 oxygen homeostasis
GO:0043524 negative regulation of neuron apoptosis
GO:0051289 protein homotetramerization
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Superoxide Dismutase (MN) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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