STAT3 antibody

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Anti STAT3 (C-Terminal) Antibody gallery image 1 — Immunohistochemical staining of paraffin embedded mouse thymus using Goat anti STAT3 antibody (**AHP1076**) at 4μgml-1 following heat mediated antigen retrieval with Tris/EDTA buffer pH 9, antibody visualization is by HRP-staining and DAB development

Goat anti STAT3 (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP1076	C* E P WB			0.1 mg

Goat anti Mouse STAT3 antibody recognizes an epitope within the C-terminal (CT) region of signal transducer and activator of transcription 3 (STAT3), an 80-85 kDa member of the STAT protein family, which plays an important role in cell growth, survival differentiation and transformation, transducing signals from cytokine receptors to the nucleus.

STAT3 is phosphorylated in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. It is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human STAT3 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02%	Sodium Azide
0.5%	Bovine Serum Albumin

Immunogen

Peptide sequence DMELTSECARSPM corresponding to the C-terminal region of STAT3 (NP_003141.2; NP_644805.1).

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P40763 Related reagents
Entrez Gene: STAT3 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0000122 negative regulation of transcription from RNA polymerase II promoter; GO:0005509 calcium ion binding; GO:0001659 temperature homeostasis; GO:0001754 eye photoreceptor cell differentiation; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0006606 protein import into nucleus; GO:0005829 cytosol; GO:0004871 signal transducer activity; GO:0005634 nucleus; GO:0006928 cellular component movement; GO:0008134 transcription factor binding; GO:0016563 transcription activator activity; GO:0019901 protein kinase binding; GO:0019953 sexual reproduction; GO:0032355 response to estradiol stimulus; GO:0042593 glucose homeostasis; GO:0042755 eating behavior; GO:0044419 interspecies interaction between organisms; GO:0046983 protein dimerization activity; GO:0048011 nerve growth factor receptor signaling pathway; GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway; GO:0070102 interleukin-6-mediated signaling pathway
Regulatory: For research purposes only

Applications of STAT3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		1/32,000
Immunohistology - Frozen ¹		10ug/ml
Immunohistology - Paraffin
Western Blotting	0	5ug/ml

¹Overnight staining is recommended for this antibody

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Mouse Thymus. .
Western Blotting: Goat anti STAT3 antibody detects a band of approximately 80-85kDa in A431 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	1 mg	F
Rabbit anti Goat IgG (Fc):HRP	STAR122P	1 mg	C E WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Histar Detection System	STAR3000B	150 Tests	C P