STAT3 antibody
STAT3 is phosphorylated in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. It is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human STAT3 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence DMELTSECARSPM corresponding to the C-terminal region of STAT3 (NP_003141.2; NP_644805.1).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P40763
- Entrez Gene
- STAT3
- GO Terms
- GO:0005886 plasma membrane
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0005509 calcium ion binding
- GO:0001659 temperature homeostasis
- GO:0001754 eye photoreceptor cell differentiation
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0006606 protein import into nucleus
- GO:0005829 cytosol
- GO:0004871 signal transducer activity
- GO:0005634 nucleus
- GO:0006928 cellular component movement
- GO:0008134 transcription factor binding
- GO:0016563 transcription activator activity
- GO:0019901 protein kinase binding
- GO:0019953 sexual reproduction
- GO:0032355 response to estradiol stimulus
- GO:0042593 glucose homeostasis
- GO:0042755 eating behavior
- GO:0044419 interspecies interaction between organisms
- GO:0046983 protein dimerization activity
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
- GO:0070102 interleukin-6-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of STAT3 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/32,000 | ||
Immunohistology - Frozen 1 | 10ug/ml | ||
Immunohistology - Paraffin | |||
Western Blotting | 0 | 5ug/ml |
- 1Overnight staining is recommended for this antibody
- Histology Positive Control Tissue
- Mouse Thymus. .
- Western Blotting
- Goat anti STAT3 antibody detects a band of approximately 80-85kDa in A431 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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Product Specific References
Further Reading
-
Levy, D.E. & Lee, C.K. (2002) What does Stat3 do?
J Clin Invest. 109 (9): 1143-8.
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