STAT3 antibody
Goat anti Mouse STAT3 antibody recognizes an epitope within the C-terminal (CT) region of signal transducer and activator of transcription 3 (STAT3), an 80-85 kDa member of the STAT protein family, which plays an important role in cell growth, survival differentiation and transformation, transducing signals from cytokine receptors to the nucleus.
STAT3 is phosphorylated in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. It is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
STAT3 is phosphorylated in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukaemia inhibitory factor. It is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human STAT3 (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence DMELTSECARSPM corresponding to the C-terminal region of STAT3 (NP_003141.2; NP_644805.1).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P40763
- Entrez Gene
- STAT3
- GO Terms
- GO:0005886 plasma membrane
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0005509 calcium ion binding
- GO:0001659 temperature homeostasis
- GO:0001754 eye photoreceptor cell differentiation
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0006606 protein import into nucleus
- GO:0005829 cytosol
- GO:0004871 signal transducer activity
- GO:0005634 nucleus
- GO:0006928 cellular component movement
- GO:0008134 transcription factor binding
- GO:0016563 transcription activator activity
- GO:0019901 protein kinase binding
- GO:0019953 sexual reproduction
- GO:0032355 response to estradiol stimulus
- GO:0042593 glucose homeostasis
- GO:0042755 eating behavior
- GO:0044419 interspecies interaction between organisms
- GO:0046983 protein dimerization activity
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0060397 JAK-STAT cascade involved in growth hormone signaling pathway
- GO:0070102 interleukin-6-mediated signaling pathway
- Regulatory
- For research purposes only
Applications of STAT3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/32,000 | ||
| Immunohistology - Frozen 1 | 10ug/ml | ||
| Immunohistology - Paraffin | |||
| Western Blotting | 0.5 | 5ug/ml |
- 1Overnight staining is recommended for this antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Mouse Thymus. .
- Western Blotting
- Goat anti STAT3 antibody detects a band of approximately 80-85kDa in A431 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
Further Reading
-
Levy, D.E. & Lee, C.K. (2002) What does Stat3 do?
J Clin Invest. 109 (9): 1143-8.
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