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SQSTM1 antibody

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Anti SQSTM1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti SQSTM1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti SQSTM1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti SQSTM1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Rabbit anti Human SQSTM1 antibody (VPA00675) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to SQSTM1 (molecular weight 60 kDa).
Anti SQSTM1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against SQSTM1 (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti SQSTM1 antibody (VPA00675) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 300 second (chemiluminescent channel) and 60 second (rhodamine channel) exposure.
Anti SQSTM1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of SQSTM1 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against SQSTM1 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

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Rabbit anti SQSTM1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
SQSTM1

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Product Code Applications Pack Size List Price Your Price Qty
VPA00675
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Rabbit anti Human SQSTM1 antibody recognizes sequestosome-1, also known as EBI3-associated protein of 60 kDa, EBI3-associated protein p60, EBIAP or ubiquitin-binding protein p62.

The SQSTM1 gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for SQSTM1. Mutations in SQSTM1 gene result in sporadic and familial Paget disease of bone (provided by RefSeq, Mar 2009).

Rabbit anti Human SQSTM1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti SQSTM1 detects a band of approximately 60 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the middle region of human SQSTM1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml loader
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loader
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
Q13501
Entrez Gene
SQSTM1
GO Terms
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0008104 protein localization
GO:0005080 protein kinase C binding
GO:0005654 nucleoplasm
GO:0005770 late endosome
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006916 anti-apoptosis
GO:0030971 receptor tyrosine kinase binding
GO:0008270 zinc ion binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0016197 endosome transport
GO:0016236 macroautophagy
GO:0030154 cell differentiation
GO:0042169 SH2 domain binding
GO:0043122 regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043130 ubiquitin binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048011 nerve growth factor receptor signaling pathway

VPA00675

161118

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