SQSTM1 antibody

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Rabbit anti SQSTM1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00675
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human SQSTM1 antibody recognizes sequestosome-1, also known as EBI3-associated protein of 60 kDa, EBI3-associated protein p60, EBIAP or ubiquitin-binding protein p62.

The SQSTM1 gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for SQSTM1. Mutations in SQSTM1 gene result in sporadic and familial Paget disease of bone (provided by RefSeq, Mar 2009).

Rabbit anti Human SQSTM1 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti SQSTM1 detects a band of approximately 60 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the middle region of human SQSTM1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q13501
Entrez Gene
SQSTM1
GO Terms
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0008104 protein localization
GO:0005080 protein kinase C binding
GO:0005654 nucleoplasm
GO:0005770 late endosome
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0006916 anti-apoptosis
GO:0030971 receptor tyrosine kinase binding
GO:0008270 zinc ion binding
GO:0008624 induction of apoptosis by extracellular signals
GO:0016197 endosome transport
GO:0016236 macroautophagy
GO:0030154 cell differentiation
GO:0042169 SH2 domain binding
GO:0043122 regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043130 ubiquitin binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048011 nerve growth factor receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of SQSTM1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml