Goat anti Human SHH antibody
recognizes Sonic hedgehog protein (SHH), also known as HHG-1 and ShhNC.
SHH is the central protein in the Sonic hedgehog (Shh) signaling cascade, which regulates development of multicellular embryos. More specifically, SHH is a polarizing morphogen, inducing different cell fates at different thresholds of SHH concentration. In this way, SHH specifies the correct pattern of cell specialization along developing vertebrate limbs and the neural tube (Choudhry et al.. 2014
), and therefore, SHH is crucial for correct embryonic development (Tickle and Towers 2017
). SHH is widely-expressed in post-embryonic tissues, and plays an important role in tissue regeneration and repair (Choudhry et al. 2014
). Tight regulation of SHH is important because the protein is oncogenic, and abnormal SHH signaling is a hallmark of many cancers. Furthermore, tumorigenesis, tumor progression and therapeutic responses are all affected by the SHH signaling pathway (Rimkus et al.. 2016
), which is consistent with evidence that embryogenesis and tumorigenesis share common characteristics, both depending on coordinated mechanisms of proliferation, differentiation, and migration (Carballo et al.. 2018
). Inhibitors of SMO, a downstream target of SHH, are FDA-approved for treatment of basal cell carcinoma (Carpenter and Ray 2019
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Shh antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Goat anti Human SHH detects a band of approximately 48 kDa in cell lysates.