Shh antibody
Goat anti Human SHH antibody recognizes Sonic hedgehog protein (SHH), also known as HHG-1 and ShhNC.
SHH is the central protein in the Sonic hedgehog (Shh) signaling cascade, which regulates development of multicellular embryos. More specifically, SHH is a polarizing morphogen, inducing different cell fates at different thresholds of SHH concentration. In this way, SHH specifies the correct pattern of cell specialization along developing vertebrate limbs and the neural tube (Choudhry et al.. 2014), and therefore, SHH is crucial for correct embryonic development (Tickle and Towers 2017). SHH is widely-expressed in post-embryonic tissues, and plays an important role in tissue regeneration and repair (Choudhry et al. 2014). Tight regulation of SHH is important because the protein is oncogenic, and abnormal SHH signaling is a hallmark of many cancers. Furthermore, tumorigenesis, tumor progression and therapeutic responses are all affected by the SHH signaling pathway (Rimkus et al.. 2016), which is consistent with evidence that embryogenesis and tumorigenesis share common characteristics, both depending on coordinated mechanisms of proliferation, differentiation, and migration (Carballo et al.. 2018). Inhibitors of SMO, a downstream target of SHH, are FDA-approved for treatment of basal cell carcinoma (Carpenter and Ray 2019).
SHH is the central protein in the Sonic hedgehog (Shh) signaling cascade, which regulates development of multicellular embryos. More specifically, SHH is a polarizing morphogen, inducing different cell fates at different thresholds of SHH concentration. In this way, SHH specifies the correct pattern of cell specialization along developing vertebrate limbs and the neural tube (Choudhry et al.. 2014), and therefore, SHH is crucial for correct embryonic development (Tickle and Towers 2017). SHH is widely-expressed in post-embryonic tissues, and plays an important role in tissue regeneration and repair (Choudhry et al. 2014). Tight regulation of SHH is important because the protein is oncogenic, and abnormal SHH signaling is a hallmark of many cancers. Furthermore, tumorigenesis, tumor progression and therapeutic responses are all affected by the SHH signaling pathway (Rimkus et al.. 2016), which is consistent with evidence that embryogenesis and tumorigenesis share common characteristics, both depending on coordinated mechanisms of proliferation, differentiation, and migration (Carballo et al.. 2018). Inhibitors of SMO, a downstream target of SHH, are FDA-approved for treatment of basal cell carcinoma (Carpenter and Ray 2019).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human SHH were raised by repeated immunizations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
0.5% Bovine Serum Albumin - Immunogen
- Peptide with sequence C-DSEALHPLGMAVK, from the C Terminus of the protein sequence according to NP_000184.1.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Shh antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 3 µg/ml | 0.5 µg/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Goat anti Human SHH detects a band of approximately 48 kDa in cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
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| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
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