Goat anti Human SCRIB antibody
recognizes protein scribble homolog, also known as Scribble, CRIB1, KIAA0147, LAP4, SCRB1, and VARTUL.
SCRIB is a scaffold protein responsible for correct apical-basal cell polarity of the epithelial tissues (Kapil et al. 2017
). Appropriate polarity is important for cell migration and morphogenesis during development, and for wound healing in adult tissue. SCRIB also plays an important role in preventing cancer progression. Loss of SCRIB function disrupts apical-basal polarity and junctional integrity, and causes inappropriate proliferation which results in overgrowth of tissues. Overexpression of SCRIB has been found in tumors of the colon, breast, lung, ovary and prostate (Ono et al. 2015
). Correct SCRIB function depends on its localization to the plasma membrane, with mislocalization promoting invasion of cells through the extracellular matrix (Sun et al. 2017
). Accordingly, SCRIB can be found in the cytoplasm in some tumor types, and even in the nucleus in hepatocellular carcinoma and liver tumors (Kapil et al. 2017
). Within neurological studies of Drosophila, SCRIB has been found to orchestrate an intracellular signaling process for forgetting information (Cervantes-Sandoval et al. 2016
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Scrib antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Goat anti Human SCRIB antibody detects a band of approximately 200 to 250 kDa in cell lysates.