ROCK2 antibody

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Rabbit anti ROCK2

ROCK2 Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00734 WB 100 µl
VPA00734KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human ROCK2 antibody recognizes Rho-associated protein kinase 2, also known as p164 ROCK-2, or rho-associated coiled-coil-containing protein kinase II.

The protein encoded by ROCK2 gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho (provided by RefSeq, Jul 2008).

Rabbit anti Human ROCK2 antibody detects a band of 160 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Buffer Solution
HEPES buffered saline
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum albumin
<50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum albumin
<50% Glycerol
Immunogen
Synthetic peptide of human ROCK2

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O75116 Related reagents
Entrez Gene
ROCK2 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0000910 cytokinesis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005198 structural molecule activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0007411 axon guidance
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ROCK2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Rabbit anti ROCK2 detects a band of approximately 160 kDa in HeLa cell lysates
Western Blotting
Rabbit anti ROCK2 detects a band of approximately 160 kDa in HeLa cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB