ROCK1 antibody

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Rabbit anti ROCK1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00923
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human ROCK1 antibody recognizes ROCK1, also known as P160ROCK and rho-associated protein kinase 1.

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18 (provided by RefSeq, Aug 2015).

Rabbit anti Human ROCK1 antibody detects a band of 160 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti ROCK1 antibody recognizes a band of approximately 160 kDa in Jurkat cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human ROCK1

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q13464
Entrez Gene
ROCK1
GO Terms
GO:0000139 Golgi membrane
GO:0000910 cytokinesis
GO:0030036 actin cytoskeleton organization
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0005814 centriole
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007266 Rho protein signal transduction
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0022614 membrane to membrane docking
GO:0050901 leukocyte tethering or rolling
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of ROCK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml