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RIG-I antibody

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Anti RIG-I Antibody thumbnail image 1 Anti RIG-I Antibody thumbnail image 2
Anti RIG-I Antibody gallery image 1
Western blot analysis of whole cell lysate from C2C12 mouse myoblast cells probed with Rabbit anti Human RIG-1 (AHP1776) at 0.5(A), 1(B) and 2(C) ug/ml.
Anti RIG-I Antibody gallery image 2
Immunohistochemical staining of human heart with Rabbit anti Human RIG-1 (AHP1776).

Rabbit anti RIG-I

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
RIG-I

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Product Code Applications Pack Size List Price Your Price Qty
AHP1776
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti RIG-I antibody recognizes human Retinoic acid-inducible gene 1 protein (RIG-I), also known as RIG-1 or DEAD-box protein 58. RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human RIG-I were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Human GST-tagged RIG-1 protein.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5.0ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human heart
Western Blotting
AHP1776 detects a band of approximately 84 kDa in C2C12cell lysate.

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for RIG-I antibody

  1. Satoh, T. et al. (2014) Tumor microenvironment and RIG-I signaling molecules in Epstein Barr virus-positive and -negative classical Hodgkin lymphoma of the elderly.
    J Clin Exp Hematop. 54 (1): 75-84.

Further Reading

  1. Akira, S. et al. (2006) Pathogen recognition and innate immunity.
    Cell. 124 (4): 783-801.
  2. Yoneyama, M. et al. (2004) The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses.
    Nat Immunol. 5 (7): 730-7.
  3. Sharma S, et al (2003) Triggering the interferon antiviral response through an IKK-related pathway
    Science 300:1148-51.
  4. Alexopoulou, L. et al. (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
    Nature. 413 (6857): 732-8.

RRID
AB_2175702
UniProt
O95786
Entrez Gene
DDX58
GO Terms
GO:0005515 protein binding
GO:0002230 positive regulation of defense response to virus by host
GO:0003723 RNA binding
GO:0009615 response to virus
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0008026 ATP-dependent helicase activity
GO:0008270 zinc ion binding
GO:0032480 negative regulation of type I interferon production
GO:0032727 positive regulation of interferon-alpha production
GO:0032728 positive regulation of interferon-beta production
GO:0045087 innate immune response

AHP1776

150418 157978

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