Rabbit anti RIG-I antibody recognizes human Retinoic acid-inducible gene 1 protein (RIG-I), also known as RIG-1 or DEAD-box protein 58. RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells. Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES. RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human RIG-I were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Human GST-tagged RIG-1 protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- O95786 Related reagents
- Entrez Gene
- DDX58 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0002230 positive regulation of defense response to virus by host
- GO:0003723 RNA binding
- GO:0009615 response to virus
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0008026 ATP-dependent helicase activity
- GO:0008270 zinc ion binding
- GO:0032480 negative regulation of type I interferon production
- GO:0032727 positive regulation of interferon-alpha production
- GO:0032728 positive regulation of interferon-beta production
- GO:0045087 innate immune response
- For research purposes only
Applications of RIG-I antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Paraffin 1||5.0ug/ml|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human heart
- Western Blotting
- AHP1776 detects a band of approximately 84 kDa in C2C12cell lysate.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Antigen Retrieval Buffer, pH8.0||BUF025A||500 ml||P|
|Antigen Retrieval Buffer, pH8.0||BUF025C||50 ml||P|
|Histar Detection System||STAR3000A||50 Tests||C P|
|TidyBlot™ Western Blot Detection Reagent:HRP||STAR209P||0.5 ml||WB*|
Product Specific References
References for RIG-I antibody
Satoh, T. et al. (2014) Tumor microenvironment and RIG-I signaling molecules in Epstein Barr virus-positive and -negative classical Hodgkin lymphoma of the elderly.
J Clin Exp Hematop. 54 (1): 75-84.
Akira, S. et al. (2006) Pathogen recognition and innate immunity.
Cell. 124 (4): 783-801.
Yoneyama, M. et al. (2004) The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses.
Nat Immunol. 5 (7): 730-7.
Sharma S, et al (2003) Triggering the interferon antiviral response through an IKK-related pathway
Alexopoulou, L. et al. (2001) Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3.
Nature. 413 (6857): 732-8.