PSMC2 antibody

ISO 9001 Quality Assurance Manufacturing | Print

siRNA Validated

Anti PSMC2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti PSMC2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti PSMC2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti PSMC2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Rabbit anti Human PSMC2 antibody (**VPA00709**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 49 second exposure. Arrow points to PSMC2 (molecular weight 47 kDa).

Anti PSMC2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMC2 (1 & 2) or a scrambled siRNA (-) probed with Rabbit anti PSMC2 antibody (**VPA00709**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 1705046) and hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 107 second (chemiluminescent channel) and 30 second exposure.

Anti PSMC2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of PSMC2 from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against PSMC2 (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the tubulin signal.

Filter by Application:

Rabbit anti PSMC2

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PSMC2

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00709 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Rabbit anti Human PSMC2 antibody recognizes the 26S protease regulatory subunit 7, also known as 26S proteasome AAA-ATPase subunit RPT1, mammalian suppressor of sgv-1 of yeast, proteasome 26S subunit ATPase 2, proteasome 26S subunit, ATPase 2.

The PSMC2 gene encodes one of the ATPase subunits of the 26S Proteasome, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with several of the basal transcription factors so, in addition to participation in proteasome functions, this subunit may participate in the regulation of transcription. This subunit may also compete with PSMC3 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Mar 2011).

Rabbit anti Human PSMC2 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti PSMC2 detects a band of approximately 47 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide.
Immunogen: KLH conjugated partial synthetic peptide of human PSMC2 (amino acids 1 - 30)
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P35998
Entrez Gene: PSMC2
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0000084 S phase of mitotic cell cycle; GO:0005515 protein binding; GO:0000216 M/G1 transition of mitotic cell cycle; GO:0000502 proteasome complex; GO:0006915 apoptosis; GO:0005739 mitochondrion; GO:0005524 ATP binding; GO:0005634 nucleus; View More GO Terms; GO:0006521 regulation of cellular amino acid metabolic process; GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; GO:0016887 ATPase activity; GO:0016032 viral reproduction; GO:0016071 mRNA metabolic process; GO:0042981 regulation of apoptosis; GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; GO:0044419 interspecies interaction between organisms; GO:0051436 negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle