PRKAR2A antibody

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Rabbit anti PRKAR2A

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00905
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human PRKAR2A antibody recognizes the PRKAR2A, also known as cAMP-dependent protein kinase regulatory subunit RII alpha and cAMP-dependent protein kinase type II-alpha regulatory subunit.

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by PRKAR2A is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) (provided by RefSeq, Jul 2008).

Rabbit anti Human PRKAR2A antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PRKAR2A antibody recognizes a band of approximately 50 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human PRKAR2A

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P13861
Entrez Gene
PRKAR2A
GO Terms
GO:0001932 regulation of protein phosphorylation
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0035556 intracellular signal transduction
GO:0005624 membrane fraction
GO:0006112 energy reserve metabolic process
GO:0006833 water transport
GO:0007202 activation of phospholipase C activity
GO:0050796 regulation of insulin secretion
GO:0008603 cAMP-dependent protein kinase regulator activity
GO:0030552 cAMP binding
GO:0034199 activation of protein kinase A activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0055085 transmembrane transport
GO:0071377 cellular response to glucagon stimulus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PRKAR2A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml