PRKAR2A antibody

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Anti PRKAR2A Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti PRKAR2A Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti PRKAR2A Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti PRKAR2A Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PRKAR2A antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to PRKAR2A (molecular weight 50 kDa)

Anti PRKAR2A Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of PRKAR2A knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti PRKAR2A antibody (**VPA00905**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 231 second and (B) 30 second exposure.

Anti PRKAR2A Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of PRKAR2A from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Rabbit anti PRKAR2A

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PRKAR2A

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00905 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human PRKAR2A antibody recognizes the PRKAR2A, also known as cAMP-dependent protein kinase regulatory subunit RII alpha and cAMP-dependent protein kinase type II-alpha regulatory subunit.

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by PRKAR2A is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER) (provided by RefSeq, Jul 2008).

Rabbit anti Human PRKAR2A antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PRKAR2A antibody recognizes a band of approximately 50 kDa in HepG2 cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
<50% Glycerol
Immunogen: Recombinant protein of human PRKAR2A
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P13861
Entrez Gene: PRKAR2A
GO Terms: GO:0001932 regulation of protein phosphorylation; GO:0007596 blood coagulation; GO:0005829 cytosol; GO:0035556 intracellular signal transduction; GO:0005624 membrane fraction; GO:0006112 energy reserve metabolic process; GO:0006833 water transport; GO:0007202 activation of phospholipase C activity; GO:0050796 regulation of insulin secretion; View More GO Terms; GO:0008603 cAMP-dependent protein kinase regulator activity; GO:0030552 cAMP binding; GO:0034199 activation of protein kinase A activity; GO:0048011 nerve growth factor receptor signaling pathway; GO:0055085 transmembrane transport; GO:0071377 cellular response to glucagon stimulus