PPP2CA antibody

ISO 9001 Quality Assurance Manufacturing | Print

KO Validated

Anti PPP2CA Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti PPP2CA Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti PPP2CA Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti PPP2CA Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PPP2CA antibody (**VPA00706**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to PPP2CA (molecular weight 36 kDa).

Anti PPP2CA Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of PPP2CA knockout 293T and wild type 293T (WT) whole cell lysates probed with Rabbit anti PPP2CA antibody (**VPA00706**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and visualized on the ChemiDoc MP with a 10 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.

Anti PPP2CA Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of PPP2CA from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Filter by Application:

Rabbit anti PPP2CA

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PPP2CA

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00706 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Rabbit anti Human PPP2CA antibody recognizes the serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, also known as PP2C-alpha, protein phosphatase 2A catalytic subunit, alpha isoform, or replication protein C.

The PPP2CA gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. PPP2CA encodes an alpha isoform of the catalytic subunit (provided by RefSeq, Jul 2008).

Rabbit anti Human PPP2CA antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti PPP2CA detects a band of approximately 36 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.01% Thiomersal
<50% Glycerol
Immunogen: Carrier-protein conjugated synthetic peptide encompassing part of the C-terminal region of human PPP2CA
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P67775
Entrez Gene: PPP2CA
GO Terms: GO:0000159 protein phosphatase type 2A complex; GO:0000188 inactivation of MAPK activity; GO:0000775 chromosome, centromeric region; GO:0000922 spindle pole; GO:0005739 mitochondrion; GO:0005829 cytosol; GO:0004721 phosphoprotein phosphatase activity; GO:0046872 metal ion binding; GO:0005625 soluble fraction; View More GO Terms; GO:0005634 nucleus; GO:0006275 regulation of DNA replication; GO:0006470 protein dephosphorylation; GO:0006672 ceramide metabolic process; GO:0006917 induction of apoptosis; GO:0007126 meiosis; GO:0008380 RNA splicing; GO:0010033 response to organic substance; GO:0010719 negative regulation of epithelial to mesenchymal transition; GO:0019932 second-messenger-mediated signaling; GO:0030111 regulation of Wnt receptor signaling pathway; GO:0030155 regulation of cell adhesion; GO:0030308 negative regulation of cell growth; GO:0042518 negative regulation of tyrosine phosphorylation of Stat3 protein; GO:0045449 regulation of transcription; GO:0071902 positive regulation of protein serine/threonine kinase activity