PPP2CA antibody

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Rabbit anti PPP2CA

PPP2CA Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00706 WB 100 µl
VPA00706KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human PPP2CA antibody recognizes the serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, also known as PP2C-alpha, protein phosphatase 2A catalytic subunit, alpha isoform, or replication protein C.

The PPP2CA gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. PPP2CA encodes an alpha isoform of the catalytic subunit (provided by RefSeq, Jul 2008).

Rabbit anti Human PPP2CA antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti PPP2CA detects a band of approximately 36 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the C-terminal region of human PPP2CA
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P67775 Related reagents
Entrez Gene
PPP2CA Related reagents
GO Terms
GO:0000159 protein phosphatase type 2A complex
GO:0000188 inactivation of MAPK activity
GO:0000775 chromosome, centromeric region
GO:0000922 spindle pole
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0004721 phosphoprotein phosphatase activity
GO:0046872 metal ion binding
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0006275 regulation of DNA replication
GO:0006470 protein dephosphorylation
GO:0006672 ceramide metabolic process
GO:0006917 induction of apoptosis
GO:0007126 meiosis
GO:0008380 RNA splicing
GO:0010033 response to organic substance
GO:0010719 negative regulation of epithelial to mesenchymal transition
GO:0019932 second-messenger-mediated signaling
GO:0030111 regulation of Wnt receptor signaling pathway
GO:0030155 regulation of cell adhesion
GO:0030308 negative regulation of cell growth
GO:0042518 negative regulation of tyrosine phosphorylation of Stat3 protein
GO:0045449 regulation of transcription
GO:0071902 positive regulation of protein serine/threonine kinase activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PPP2CA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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