POLD1 antibody
Rabbit anti Human POLD1 antibody recognizes POLD1, also known as CDC2 homolog, DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, MDPL and catalytic subunit 125 kDa.
POLD1 encodes the 125 kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for POLD1, and a pseudogene of POLD1 is located on the long arm of chromosome 6 (provided by RefSeq, Mar 2012).
Rabbit anti Human POLD1 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
POLD1 encodes the 125 kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for POLD1, and a pseudogene of POLD1 is located on the long arm of chromosome 6 (provided by RefSeq, Mar 2012).
Rabbit anti Human POLD1 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti POLD1 antibody recognizes a band of approximately 130 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
<50% Glycerol - Immunogen
- Recombinant protein of human POLD1
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P28340
- Entrez Gene
- POLD1
- GO Terms
- GO:0000084 S phase of mitotic cell cycle
- GO:0000166 nucleotide binding
- GO:0005515 protein binding
- GO:0000722 telomere maintenance via recombination
- GO:0000731 DNA synthesis involved in DNA repair
- GO:0003677 DNA binding
- GO:0003682 chromatin binding
- GO:0003887 DNA-directed DNA polymerase activity
- GO:0004527 exonuclease activity
- GO:0046872 metal ion binding
- GO:0005654 nucleoplasm
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006284 base-excision repair
- GO:0006297 nucleotide-excision repair, DNA gap filling
- GO:0009411 response to UV
- GO:0032201 telomere maintenance via semi-conservative replication
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of POLD1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
