POLD1 antibody

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Anti POLD1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with POLD1 antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to POLD1 (molecular weight 130 kDa)

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Rabbit anti POLD1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: POLD1

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VPA00840 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human POLD1 antibody recognizes POLD1, also known as CDC2 homolog, DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, MDPL and catalytic subunit 125 kDa.

POLD1 encodes the 125 kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for POLD1, and a pseudogene of POLD1 is located on the long arm of chromosome 6 (provided by RefSeq, Mar 2012).

Rabbit anti Human POLD1 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti POLD1 antibody recognizes a band of approximately 130 kDa in K562 cell lysates
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
<50% Glycerol
Immunogen: Recombinant protein of human POLD1
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P28340
Entrez Gene: POLD1
GO Terms: GO:0000084 S phase of mitotic cell cycle; GO:0000166 nucleotide binding; GO:0005515 protein binding; GO:0000722 telomere maintenance via recombination; GO:0000731 DNA synthesis involved in DNA repair; GO:0003677 DNA binding; GO:0003682 chromatin binding; GO:0003887 DNA-directed DNA polymerase activity; GO:0004527 exonuclease activity; View More GO Terms; GO:0046872 metal ion binding; GO:0005654 nucleoplasm; GO:0006271 DNA strand elongation involved in DNA replication; GO:0006283 transcription-coupled nucleotide-excision repair; GO:0006284 base-excision repair; GO:0006297 nucleotide-excision repair, DNA gap filling; GO:0009411 response to UV; GO:0032201 telomere maintenance via semi-conservative replication