POLD1 antibody
POLD1 encodes the 125 kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for POLD1, and a pseudogene of POLD1 is located on the long arm of chromosome 6 (provided by RefSeq, Mar 2012).
Rabbit anti Human POLD1 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti POLD1 antibody recognizes a band of approximately 130 kDa in K562 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography on immunogen
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
<50% Glycerol - Immunogen
- Recombinant protein of human POLD1
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P28340
- Entrez Gene
- POLD1
- GO Terms
- GO:0000084 S phase of mitotic cell cycle
- GO:0000166 nucleotide binding
- GO:0005515 protein binding
- GO:0000722 telomere maintenance via recombination
- GO:0000731 DNA synthesis involved in DNA repair
- GO:0003677 DNA binding
- GO:0003682 chromatin binding
- GO:0003887 DNA-directed DNA polymerase activity
- GO:0004527 exonuclease activity
- GO:0046872 metal ion binding
- GO:0005654 nucleoplasm
- GO:0006271 DNA strand elongation involved in DNA replication
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006284 base-excision repair
- GO:0006297 nucleotide-excision repair, DNA gap filling
- GO:0009411 response to UV
- GO:0032201 telomere maintenance via semi-conservative replication
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of POLD1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (H/L):HRP | STAR208P | WB | 2 ml |
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