POLD1 antibody

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Rabbit anti POLD1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00840
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human POLD1 antibody recognizes POLD1, also known as CDC2 homolog, DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, MDPL and catalytic subunit 125 kDa.

POLD1 encodes the 125 kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for POLD1, and a pseudogene of POLD1 is located on the long arm of chromosome 6 (provided by RefSeq, Mar 2012).

Rabbit anti Human POLD1 antibody detects a band of 130 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti POLD1 antibody recognizes a band of approximately 130 kDa in K562 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human POLD1

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P28340
Entrez Gene
POLD1
GO Terms
GO:0000084 S phase of mitotic cell cycle
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000722 telomere maintenance via recombination
GO:0000731 DNA synthesis involved in DNA repair
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003887 DNA-directed DNA polymerase activity
GO:0004527 exonuclease activity
GO:0046872 metal ion binding
GO:0005654 nucleoplasm
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006284 base-excision repair
GO:0006297 nucleotide-excision repair, DNA gap filling
GO:0009411 response to UV
GO:0032201 telomere maintenance via semi-conservative replication
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of POLD1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml