PDGF-B antibody

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Rabbit anti PDGF-B

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00534
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human PDGF-B antibody recognizes platelet-derived growth factor subunit B, also known as PDGF subunit B, becaplermin, platelet-derived growth factor 2, platelet-derived growth factor beta polypeptide or proto-oncogene c-Sis.

The protein encoded by the PDGFB gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimer chains are connected by disulfide bonds.

Mutations in PDGFB are associated with meningioma and Basal ganglia calcification, idiopathic, 5 (IBGC5), an autosomal dominant disorder characterized by progressive neurologic symptoms associated with brain calcifications (Keller et al. 2013). Reciprocal translocations between chromosomes 22 and 17, at sites where PDGFB and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans (DFSB) resulting from unregulated expression of growth factor (Kiuru-Kuhlefelt et al. 2001). Two alternatively spliced transcript variants encoding different isoforms have been identified for PDGFB (provided by RefSeq, Feb 2013).

Rabbit anti Human PDGF-B antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PDGF-B detects a band of approximately 27 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the N terminal region of human PDGF-B

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P01127
Entrez Gene
PDGFB
GO Terms
GO:0000139 Golgi membrane
GO:0001938 positive regulation of endothelial cell proliferation
GO:0002548 monocyte chemotaxis
GO:0002576 platelet degranulation
GO:0005576 extracellular region
GO:0005161 platelet-derived growth factor receptor binding
GO:0005518 collagen binding
GO:0005788 endoplasmic reticulum lumen
GO:0008083 growth factor activity
GO:0009986 cell surface
GO:0010512 negative regulation of phosphatidylinositol biosynthetic process
GO:0010544 negative regulation of platelet activation
GO:0014911 positive regulation of smooth muscle cell migration
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0042803 protein homodimerization activity
GO:0043406 positive regulation of MAP kinase activity
GO:0043498 cell surface binding
GO:0043536 positive regulation of blood vessel endothelial cell migration
GO:0045740 positive regulation of DNA replication
GO:0046982 protein heterodimerization activity
GO:0048008 platelet-derived growth factor receptor signaling pathway
GO:0048146 positive regulation of fibroblast proliferation
GO:0048407 platelet-derived growth factor binding
GO:0050921 positive regulation of chemotaxis
GO:0051781 positive regulation of cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of PDGF-B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml