PDGF-B antibody

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Anti PDGF-B Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PDGF-B antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 15 second exposure. Arrow points to PDGF-B (molecular weight 27 kDa).

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Rabbit anti PDGF-B

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PDGF-B

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Rabbit anti Human PDGF-B antibody recognizes platelet-derived growth factor subunit B, also known as PDGF subunit B, becaplermin, platelet-derived growth factor 2, platelet-derived growth factor beta polypeptide or proto-oncogene c-Sis.

The protein encoded by the PDGFB gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimer chains are connected by disulfide bonds.

Mutations in PDGFB are associated with meningioma and Basal ganglia calcification, idiopathic, 5 (IBGC5), an autosomal dominant disorder characterized by progressive neurologic symptoms associated with brain calcifications (Keller et al. 2013). Reciprocal translocations between chromosomes 22 and 17, at sites where PDGFB and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans (DFSB) resulting from unregulated expression of growth factor (Kiuru-Kuhlefelt et al. 2001). Two alternatively spliced transcript variants encoding different isoforms have been identified for PDGFB (provided by RefSeq, Feb 2013).

Rabbit anti Human PDGF-B antibody detects a band of 27 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PDGF-B detects a band of approximately 27 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the N terminal region of human PDGF-B
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P01127
Entrez Gene: PDGFB
GO Terms: GO:0000139 Golgi membrane; GO:0001938 positive regulation of endothelial cell proliferation; GO:0002548 monocyte chemotaxis; GO:0002576 platelet degranulation; GO:0005576 extracellular region; GO:0005161 platelet-derived growth factor receptor binding; GO:0005518 collagen binding; GO:0005788 endoplasmic reticulum lumen; GO:0008083 growth factor activity; View More GO Terms; GO:0009986 cell surface; GO:0010512 negative regulation of phosphatidylinositol biosynthetic process; GO:0010544 negative regulation of platelet activation; GO:0014911 positive regulation of smooth muscle cell migration; GO:0030168 platelet activation; GO:0031093 platelet alpha granule lumen; GO:0042803 protein homodimerization activity; GO:0043406 positive regulation of MAP kinase activity; GO:0043498 cell surface binding; GO:0043536 positive regulation of blood vessel endothelial cell migration; GO:0045740 positive regulation of DNA replication; GO:0046982 protein heterodimerization activity; GO:0048008 platelet-derived growth factor receptor signaling pathway; GO:0048146 positive regulation of fibroblast proliferation; GO:0048407 platelet-derived growth factor binding; GO:0050921 positive regulation of chemotaxis; GO:0051781 positive regulation of cell division