NFkB p65 (pSer276) antibody
Rabbit anti NFkB p65 (pSer276) antibody recognizes nuclear factor NF-kappa-B p65 subunit (NFkB3 p65) also known as transcription factor p65 or RELA, when phosphorylated at serine 276. NFkB p65 is part of the NFkB transcription factor complex that also includes RELB, NFkB1 p105, NFkB1 p50, REL and NFkB2 p52. NFkB p65 regulates expression of a wide variety of genes involved in immune and inflammatory responses (May, M.J. & Ghosh, S. 1997). Protein kinase A (PKA) phosphorylates NFkB p65 on serine 276 and modulates transcriptional activity of NFkB by mediating its interaction with CBP/p300 (Zhong, H. et al. 1998). Serine 276 has also been demonstrated to be phosphorylated by mitogen- and stress- activated protein kinase-1 (MSK1) (Vermeulen, L. et al. 2003). The NFkB pathway plays a critical role in orchestrating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to NFkB p65 (pSer276) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 276 of human NFkB p65
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q04206
- Entrez Gene
- RELA
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003682 chromatin binding
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005829 cytosol
- GO:0005667 transcription factor complex
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0006968 cellular defense response
- GO:0008063 Toll signaling pathway
- GO:0042802 identical protein binding
- GO:0010224 response to UV-B
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0019221 cytokine-mediated signaling pathway
- GO:0019901 protein kinase binding
- GO:0031293 membrane protein intracellular domain proteolysis
- GO:0031625 ubiquitin protein ligase binding
- GO:0032582 negative regulation of gene-specific transcription
- GO:0033613 activating transcription factor binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042301 phosphate binding
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0047485 protein N-terminus binding
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050727 regulation of inflammatory response
- GO:0050852 T cell receptor signaling pathway
- GO:0051059 NF-kappaB binding
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051607 defense response to virus
- GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
- GO:0070491 repressing transcription factor binding
- GO:0070555 response to interleukin-1
- Regulatory
- For research purposes only
Applications of NFkB p65 (pSer276) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | 1/50 | 1/100 | |
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti NFkB p65 | VPA00015 | E GS IP WB | 100 µl |
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