Nestin antibody

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Rabbit anti Human Nestin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1739
Datasheet
E P* WB
MSDS
50 µg loader

Rabbit anti Human nestin antibody recognizes human Nestin, an intermediate filament involved in cytoskeletal organisation.

Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.

Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
Mouse
Bovine
Dog
Hamster
Rat
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human Nestin were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant human Nestin (aa1484-1500).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q6P5H2
Entrez Gene
Nes
GO Terms
GO:0005882 intermediate filament
GO:0005198 structural molecule activity
GO:0005737 cytoplasm
GO:0007420 brain development
GO:0019215 intermediate filament binding
GO:0030844 positive regulation of intermediate filament depolymerization
GO:0031076 embryonic camera-type eye development
GO:0043066 negative regulation of apoptosis
GO:0048858 cell projection morphogenesis
GO:2000179 positive regulation of neural precursor cell proliferation
Regulatory
For research purposes only

Applications of Nestin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20000 1/80000
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 1/500 1/3000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

References for Nestin antibody

  1. Johansen,T. et al. (2017) Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells
    Stem Cells International. 2017: 1-14.