Nestin antibody
Rabbit anti Human nestin antibody recognizes human Nestin, an intermediate filament involved in cytoskeletal organisation.
Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.
Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.
Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Macaque Mouse Bovine Dog Hamster Rat Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Human Nestin were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Recombinant human Nestin (aa1484-1500).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q6P5H2
- Entrez Gene
- Nes
- GO Terms
- GO:0005882 intermediate filament
- GO:0005198 structural molecule activity
- GO:0005737 cytoplasm
- GO:0007420 brain development
- GO:0019215 intermediate filament binding
- GO:0030844 positive regulation of intermediate filament depolymerization
- GO:0031076 embryonic camera-type eye development
- GO:0043066 negative regulation of apoptosis
- GO:0048858 cell projection morphogenesis
- GO:2000179 positive regulation of neural precursor cell proliferation
- Regulatory
- For research purposes only
Applications of Nestin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/20000 | 1/80000 | |
| Immunohistology - Paraffin 1 | 5ug/ml | ||
| Western Blotting | 1/500 | 1/3000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human kidney
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Nestin antibody
-
Johansen,T. et al. (2017) Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells
Stem Cells International. 2017: 1-14.
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