Nestin antibody

Rabbit anti Human Nestin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Nestin

Product Code Applications Pack Size List Price Your Price Qty
AHP1739
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Rabbit anti Human nestin antibody recognizes human Nestin, an intermediate filament involved in cytoskeletal organisation.

Nestin is a marker of multipotent neuroectodermal cells and is downregulated as the cells cease division and differentiate to their neural or glial lineages.

Expression of Nestin is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Macaque
Mouse
Bovine
Dog
Hamster
Rat
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human Nestin were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant human Nestin (aa1484-1500).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20000 1/80000
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 1/500 1/3000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human kidney

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

References for Nestin antibody

  1. Johansen,T. et al. (2017) Comparative Analysis of Spontaneous and Stimulus-Evoked Calcium Transients in Proliferating and Differentiating Human Midbrain-Derived Stem Cells
    Stem Cells International. 2017: 1-14.

RRID
AB_2151137
UniProt
Q6P5H2
Entrez Gene
Nes
GO Terms
GO:0005882 intermediate filament
GO:0005198 structural molecule activity
GO:0005737 cytoplasm
GO:0007420 brain development
GO:0019215 intermediate filament binding
GO:0030844 positive regulation of intermediate filament depolymerization
GO:0031076 embryonic camera-type eye development
GO:0043066 negative regulation of apoptosis
GO:0048858 cell projection morphogenesis
GO:2000179 positive regulation of neural precursor cell proliferation
View more products with NESTIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products