Myosin antibody

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Anti Human Myosin Regulatory Light Chain Antibody thumbnail image 1

Anti Human Myosin Regulatory Light Chain Antibody gallery image 1 — Staining of formalin fixed, paraffin embedded human colonic smooth muscle with Rabbit anti Human myosin regulatory light chain (**AHP1730**).

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Rabbit anti Human Myosin Regulatory Light Chain

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Myosin
Region: (REGULATORY LIGHT CHAIN)

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AHP1730 Datasheet Datasheet SDS SDS	E P * WB	50 µg		Log in	Get Quote
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Rabbit anti Human myosin regulatory light chain antibody recognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20.

Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Birds Expected from Sequence

Mammals Expected from Sequence

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to Human Myosin Regulatory Light Chain were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: A synthetic peptide corresponding to amino acids 12-27 of human Myosin Regulatory Light Chain.
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Birds	Expected from Sequence
Mammals	Expected from Sequence
Mouse
Rat

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/5000	1/20000
Immunohistology - Paraffin ¹		2.5ug/ml
Western Blotting	1/500	1/2000

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Smooth muscle

Description	Product Code	Applications	Pack Size	Your Price
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in	Get Quote
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Description	Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

Synonyms: LC20
RRID: AB_2148344
UniProt: P24844
Entrez Gene: MYL9
GO Terms: GO:0005509 calcium ion binding; GO:0003774 motor activity; GO:0006936 muscle contraction; GO:0005829 cytosol; GO:0005859 muscle myosin complex; GO:0006937 regulation of muscle contraction; GO:0007411 axon guidance; GO:0008307 structural constituent of muscle