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Myosin antibody

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Anti Human Myosin Regulatory Light Chain Antibody gallery image 1
Staining of formalin fixed, paraffin embedded human colonic smooth muscle with Rabbit anti Human myosin regulatory light chain (AHP1730).

Rabbit anti Human Myosin Regulatory Light Chain

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1730
Datasheet
E P* WB
MSDS
50 µg loader

Rabbit anti Human myosin regulatory light chain antibody recognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20.

Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Birds
Mammals
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human Myosin Regulatory Light Chain were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to amino acids 12-27 of human Myosin Regulatory Light Chain.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P24844
Entrez Gene
MYL9
GO Terms
GO:0005509 calcium ion binding
GO:0003774 motor activity
GO:0006936 muscle contraction
GO:0005829 cytosol
GO:0005859 muscle myosin complex
GO:0006937 regulation of muscle contraction
GO:0007411 axon guidance
GO:0008307 structural constituent of muscle
Regulatory
For research purposes only

Applications of Myosin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/20000
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 1/500 1/2000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Smooth muscle
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Histar Detection System STAR3000A C P 50 Tests
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