Myosin antibody
Filter by Application:
P ResetRabbit anti Human Myosin Regulatory Light Chain
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Myosin
- Region
- (REGULATORY LIGHT CHAIN)
Quick Links:
Antibody Quality and 
Unrivaled 
| Rabbit anti Human myosin regulatory light chain antibody recognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20. Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Birds Expected from Sequence Mammals Expected from Sequence Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Human Myosin Regulatory Light Chain were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide corresponding to amino acids 12-27 of human Myosin Regulatory Light Chain.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5000 | 1/20000 |
| Immunohistology - Paraffin 1 | |
2.5ug/ml | |
| Western Blotting | |
1/500 | 1/2000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Smooth muscle
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- Synonyms
- LC20
- RRID
- AB_2148344
- UniProt
- P24844
- Entrez Gene
- MYL9
- GO Terms
- GO:0005509 calcium ion binding
- GO:0003774 motor activity
- GO:0006936 muscle contraction
- GO:0005829 cytosol
- GO:0005859 muscle myosin complex
- GO:0006937 regulation of muscle contraction
- GO:0007411 axon guidance
- GO:0008307 structural constituent of muscle
View more products with MYOSIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up