Myosin antibody

100% Secure

Anti Human Myosin Regulatory Light Chain Antibody gallery image 1 — Staining of formalin fixed, paraffin embedded human colonic smooth muscle with Rabbit anti Human myosin regulatory light chain (**AHP1730**).

Rabbit anti Human Myosin Regulatory Light Chain

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1730 Datasheet	E P* WB SDS	50 µg	Log in		Get Quote

Rabbit anti Human myosin regulatory light chain antibody recognizes human myosin regulatory light chain (MRLC), also known as MYL9 and LC20.

Myosin is a hexamer composed of two high molecular weight heavy chains and four light chain subunits, which include two alkali light chains and two MRLCs. MRLC functions to stabilize the myosin heavy chain, and may also modulate the availability of cross bridges to bind to actin. Phosphorylation of the MRLC by myosin light chain kinase (MLCK) is necessary for smooth muscle contraction via activating myosin ATPase.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Birds	Expected from Sequence
Mammals	Expected from Sequence
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to Human Myosin Regulatory Light Chain were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN₃)

Immunogen

A synthetic peptide corresponding to amino acids 12-27 of human Myosin Regulatory Light Chain.

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P24844
Entrez Gene: MYL9
GO Terms: GO:0005509 calcium ion binding; GO:0003774 motor activity; GO:0006936 muscle contraction; GO:0005829 cytosol; GO:0005859 muscle myosin complex; GO:0006937 regulation of muscle contraction; GO:0007411 axon guidance; GO:0008307 structural constituent of muscle
Regulatory: For research purposes only

Applications of Myosin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/5000	1/20000
Immunohistology - Paraffin ¹		2.5ug/ml
Western Blotting	1/500	1/2000

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Smooth muscle

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in	Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in	Get Quote
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer