MED17 antibody

100% Secure


Rabbit anti MED17

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00478
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human MED17 antibody recognizes MED17 also known as, mediator of RNA polymerase II transcription subunit 17, activator-recruited cofactor 77 kDa component, cofactor required for Sp1 transcriptional activation, subunit 6, thyroid hormone receptor-associated protein complex 80 kDa component, transcriptional coactivator CRSP77 or vitamin D3 receptor-interacting protein complex 80 kDa component.

The activation of gene transcription is a multistep process triggered by factors that recognize transcriptional enhancer sites in DNA, these factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED17 gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors (provided by RefSeq, Jul 2008).

Rabbit anti Human MED17 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MED17 detects a band of approximately 95 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the N terminal region of human MED17

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9NVC6
Entrez Gene
MED17
GO Terms
GO:0004872 receptor activity
GO:0005667 transcription factor complex
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0016455 RNA polymerase II transcription mediator activity
GO:0016563 transcription activator activity
GO:0016592 mediator complex
GO:0046966 thyroid hormone receptor binding
GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity
GO:0030521 androgen receptor signaling pathway
GO:0042809 vitamin D receptor binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of MED17 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml