MED17 antibody

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Rabbit anti MED17

Rabbit anti-human MED17 antibody recognizes mediator of RNA polymerase II transcription subunit 17, also known as CRSP complex subunit 6, or activator-recruited cofactor 77 kDa component (ARC77).

MED17 Antibody with Control Lysate

Rabbit anti-human MED17 antibody recognizes mediator of RNA polymerase II transcription subunit 17, also known as CRSP complex subunit 6, or activator-recruited cofactor 77 kDa component (ARC77).

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00478 WB 100 µl
VPA00478KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human MED17 antibody recognizes MED17 also known as, mediator of RNA polymerase II transcription subunit 17, activator-recruited cofactor 77 kDa component, cofactor required for Sp1 transcriptional activation, subunit 6, thyroid hormone receptor-associated protein complex 80 kDa component, transcriptional coactivator CRSP77 or vitamin D3 receptor-interacting protein complex 80 kDa component.

The activation of gene transcription is a multistep process triggered by factors that recognize transcriptional enhancer sites in DNA, these factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED17 gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors (provided by RefSeq, Jul 2008).

Rabbit anti Human MED17 antibody detects a band of 95 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti MED17 detects a band of approximately 95 kDa in MCF7 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human MED17
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9NVC6 Related reagents
Entrez Gene
MED17 Related reagents
GO Terms
GO:0004872 receptor activity
GO:0005667 transcription factor complex
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0016455 RNA polymerase II transcription mediator activity
GO:0016563 transcription activator activity
GO:0016592 mediator complex
GO:0046966 thyroid hormone receptor binding
GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity
GO:0030521 androgen receptor signaling pathway
GO:0042809 vitamin D receptor binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of MED17 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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