Lnk antibody
Lane A: Mock transfected cells. Lane B: transfected cells.
Goat anti Human LNK antibodyrecognizes human SH2B adapter protein 3, also known as Lymphocyte-specific adapter protein Lnk or Lymphocyte adapter protein. LNK is a 575 amino acid SH2B adapter protein containing a single PH domain and a single SH2 domain. Goat anti Human LNK antibody binds to an epitope within the N-terminal region of LNK, which regulates cytokine receptor-mediated and growth factor pathways, and plays an essential role in B cell lymphopoiesis.
LNK mRNA is preferentially expressed in lymph node and spleen lymphocytes and the expression of LNK in vascular endothelial cells (ECs), increases in response to TNFα. LNK acts as a negative regulator of the pro-inflammatory molecules VCAM-1 and E-selectin induced by TNFα, modulation of P13-kinase and MAPK ERK1/2 activity. LNK is also involved in the negative regulation of Thrombopoietin-mediated cell proliferation and endomitosis in hematopoietic cell lines.
LNK mRNA is preferentially expressed in lymph node and spleen lymphocytes and the expression of LNK in vascular endothelial cells (ECs), increases in response to TNFα. LNK acts as a negative regulator of the pro-inflammatory molecules VCAM-1 and E-selectin induced by TNFα, modulation of P13-kinase and MAPK ERK1/2 activity. LNK is also involved in the negative regulation of Thrombopoietin-mediated cell proliferation and endomitosis in hematopoietic cell lines.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Antisera to LNK (NT) were raised by repeated immunisation of Goat with highly purified antigen.
Purified IgG prepared by affinity chromatography. - Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence NGPALQPSSPC from LNK amino-terminus.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9UQQ2
- Entrez Gene
- SH2B3
- GO Terms
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0004871 signal transducer activity
- Regulatory
- For research purposes only
Applications of Lnk antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 0.3 | 1.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Goat anti Human LNK antibody, directed against the N-teend of the molecule, detects a band of approximately 70 kDa in human MOLT4 cell lysates. (Predicted Mwt. 63.2 kDa). A band of 68 kDA was resolved in HUVECs (Fitau et al. 2006).
Suitable antibodies for loading controls include :
Human anti Human actin β, HCA147 or Rat anti tubulin α,MCA77G.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for Lnk antibody
-
Fitau, J. et al. (2006) The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
J Biol Chem. 281 (29): 20148-59. -
Fitau, J. et al. (2005) The adaptor protein Lnk modulates endothelial cell activation.
Nephrol Ther. 1: 228-33. -
Gery, S. et al. (2007) Adaptor protein Lnk negatively regulates the mutant MPL, MPLW515L associated with myeloproliferative disorders.
Blood. 110: 3360-4. -
Gery, S. et al. (2009) Lnk inhibits myeloproliferative disorder-associated JAK2 mutant, JAK2V617F.
J Leukoc Biol. 85: 957-65. -
Wan, M. et al. (2006) TNF-α Induces Lnk Expression Through PI3K-Dependent Signaling Pathway in Human Umbilical Vein Endothelial Cells
J Surg Res. 136: 53-7. -
Wan, M. et al. (2007) Eicosapentaenoic acid inhibits TNF-alpha-induced Lnk expression in human umbilical vein endothelial cells: involvement of the PI3K/Akt pathway.
J Nutr Biochem. 18: 17-22. -
Chatelais, M. et al. (2011) Gene transfer of the adaptor Lnk (SH2B3) prevents porcine endothelial cell activation and apoptosis: implication for xenograft's cytoprotection.
Xenotransplantation. 18: 108-20. -
Devallière, J. et al. (2012) LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets α-parvin to control cell adhesion and migration.
FASEB J. 26: 2592-606.
Further Reading
-
Tong, W. & Lodish, H.F. (2004) Lnk inhibits Tpo-mpl signaling and Tpo-mediated megakaryocytopoiesis.
J Exp Med. 200 (5): 569-80.
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