Lnk antibody

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Goat anti Human Lnk (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1003 WB 0.1 mg
Goat anti Human LNK antibodyrecognizes human SH2B adapter protein 3, also known as Lymphocyte-specific adapter protein Lnk or Lymphocyte adapter protein. LNK is a 575 amino acid SH2B adapter protein containing a single PH domain and a single SH2 domain. Goat anti Human LNK antibody binds to an epitope within the N-terminal region of LNK, which regulates cytokine receptor-mediated and growth factor pathways, and plays an essential role in B cell lymphopoiesis.

LNK mRNA is preferentially expressed in lymph node and spleen lymphocytes and the expression of LNK in vascular endothelial cells (ECs), increases in response to TNFα. LNK acts as a negative regulator of the pro-inflammatory molecules VCAM-1 and E-selectin induced by TNFα, modulation of P13-kinase and MAPK ERK1/2 activity. LNK is also involved in the negative regulation of Thrombopoietin-mediated cell proliferation and endomitosis in hematopoietic cell lines.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Antisera to LNK (NT) were raised by repeated immunisation of Goat with highly purified antigen.
Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence NGPALQPSSPC from LNK amino-terminus.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9UQQ2 Related reagents
Entrez Gene
SH2B3 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004871 signal transducer activity
Regulatory
For research purposes only

Applications of Lnk antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.3 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Goat anti Human LNK antibody, directed against the N-teend of the molecule, detects a band of approximately 70 kDa in human MOLT4 cell lysates. (Predicted Mwt. 63.2 kDa). A band of 68 kDA was resolved in HUVECs (Fitau et al. 2006).

Suitable antibodies for loading controls include :

Human anti Human actin β, HCA147 or Rat anti tubulin α,MCA77G.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Application Based External Images

Western Blotting

Product Specific References

References for Lnk antibody

  1. Fitau, J. et al. (2006) The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
    J Biol Chem. 281 (29): 20148-59.
  2. Fitau, J. et al. (2005) The adaptor protein Lnk modulates endothelial cell activation.
    Nephrol Ther. 1: 228-33.
  3. Gery, S. et al. (2007) Adaptor protein Lnk negatively regulates the mutant MPL, MPLW515L associated with myeloproliferative disorders.
    Blood. 110: 3360-4.
  4. Gery, S. et al. (2009) Lnk inhibits myeloproliferative disorder-associated JAK2 mutant, JAK2V617F.
    J Leukoc Biol. 85: 957-65.
  5. Wan, M. et al. (2006) TNF-α Induces Lnk Expression Through PI3K-Dependent Signaling Pathway in Human Umbilical Vein Endothelial Cells
    J Surg Res. 136: 53-7.
  6. Wan, M. et al. (2007) Eicosapentaenoic acid inhibits TNF-alpha-induced Lnk expression in human umbilical vein endothelial cells: involvement of the PI3K/Akt pathway.
    J Nutr Biochem. 18: 17-22.
  7. Chatelais, M. et al. (2011) Gene transfer of the adaptor Lnk (SH2B3) prevents porcine endothelial cell activation and apoptosis: implication for xenograft's cytoprotection.
    Xenotransplantation. 18: 108-20.
  8. Devallière, J. et al. (2012) LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets α-parvin to control cell adhesion and migration.
    FASEB J. 26: 2592-606.

Further Reading

  1. Tong, W. & Lodish, H.F. (2004) Lnk inhibits Tpo-mpl signaling and Tpo-mediated megakaryocytopoiesis.
    J Exp Med. 200 (5): 569-80.