Livin antibody

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Goat anti Human Livin (C-Terminal)

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.1 mg

Goat anti Human LIVIN (C terminal) antibody recognizes human Baculoviral IAP repeat-containing protein 7, also known as Kidney inhibitor of apoptosis protein, Livin, RING finger protein 50 or Melanoma inhibitor of apoptosis protein, encoded by the BIRC7 gene. Livin is a 298 amino acid, ~30kDa member of the IAP family containing a single BIR repeat and a single RING -type Zinc finger domain. Goat anti human Livin antibody, recognizes an epitope within the C-terminal (CT) region of human Livin. Two isoforms of livin are generated by alternative splicing α or isoform 2, and β or isoform 1, the latter carrying a 18 amino acid deletion toward the C-terminal region of the molecule. However, the deletion seen in Livin-β is outside the sequence used as immunogen for this Goat anti human Livin antibody which is expected to recognize both isoforms of the molecule.

Livin is upregulated in a number of cancer cell types demonstrating both nuclear and cytoplasmic localization in renal cancer cells whilst equivalent non-cancerous kidneys demonstrate only cytoplasmic staining in restricted cell types ( Wagener et al. 2007).

Product Details

Target Species
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human LIVIN (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Peptide sequence C-RAPVRSRVRT corresponding to the C-terminal region of LIVIN (UniProt: Q96CA5).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0006916 anti-apoptosis
GO:0007257 activation of JUN kinase activity
GO:0008270 zinc ion binding
GO:0019899 enzyme binding
For research purposes only

Applications of Livin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/4,000
Western Blotting 0.1ug/ml 1.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP648 detects bands of approximately 30 & 35kDa in MOLT4 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

Further Reading

  1. Vucic, D. et al. (2002) SMAC negatively regulates the anti-apoptotic activity of melanoma inhibitor of apoptosis (ML-IAP).
    J Biol Chem. 277 (14): 12275-9.

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