Kappa Light Chain antibody
Goat F(ab')2 anti Human Kappa Light Chain:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Kappa Light Chain
Antibody Quality and Performance Guarantee
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| Goat F(ab')2 anti Human Kappa Light Chain antibody recognizes human kappa light chains. This antibody has been cross absorbed with pooled human myeloma proteins with lambda light chains. |
- Target Species
- Human
- Product Form
- F(ab)2 fragments of purified IgG conjugated to Biotin - liquid
- Antiserum Preparation
- Antisera to human kappa chains were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab)2 fragments were prepared by pepsin digestion.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human kappa light chains.
- Approx. Protein Concentrations
- F(ab')2 concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/5000 | 1/20000 |
| Immunofluorescence | |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
- RRID
- AB_1628913
- UniProt
- P01834
- Entrez Gene
- IGKC
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
View more products with KAPPA LIGHT CHAIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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