IRGM antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti IRGM (Internal Region) Antibody thumbnail image 1

Anti IRGM (Internal Region) Antibody thumbnail image 2

Anti IRGM (Internal Region) Antibody gallery image 1 — Western blot analysis of human brain lysate probed with Rabbit anti Human IRGM (**AHP1887**) at 1(A) and 2(B) ug/ml.

Anti IRGM (Internal Region) Antibody gallery image 2 — Immunohistochemical staining of human brain with Rabbit anti Human IRGM (**AHP1887**).

Filter by Application:

WB P

Rabbit anti IRGM (Internal Region)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IRGM
Region: (INTERNAL REGION)

Quick Links:

Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
AHP1887 Datasheet Datasheet SDS SDS	IF P * WB	0.1 mg		Log in	Get Quote
	Log in

Rabbit anti IRGM (Internal Region) antibody recognizes human Immunity-related GTPase family M protein (IRGM), also known as LRG47.

IRGM is a putative GTPase required for clearance of bacterial and protozoan infections. It funtions in the innate immune response, most likely through regulation of autophagy.

IRGM may also function in the regulation of proinflammatory cytokine production and in macrophage adhesion and motility.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to human IRGM was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
Immunogen: Synthetic peptide corresponding to a 14 aa sequence from near the centre of human IRGM.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse
Rat

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunofluorescence		20ug/ml
Immunohistology - Paraffin ¹	5ug/ml
Western Blotting	1	2ug/ml

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human brain
Western Blotting: AHP1887 detects a band of approximately 32 kDa in human brain cell lysate.

Description	Product Code	Applications	Pack Size	Your Price
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	TidyBlot Western Blot Detection Reagent:HRP

RRID: AB_1898227
UniProt: A1A4Y4; Q6AYC2; Q60766
Entrez Gene: IRGM; Irgm1; Irgm
GO Terms: GO:0005886 plasma membrane; GO:0000139 Golgi membrane; GO:0000421 autophagic vacuole membrane; GO:0005525 GTP binding; GO:0006914 autophagy; GO:0006954 inflammatory response; GO:0016817 hydrolase activity, acting on acid anhydrides; GO:0030670 phagocytic vesicle membrane; GO:0042995 cell projection; View More GO Terms; GO:0045087 innate immune response; GO:0005783 endoplasmic reticulum; GO:0005765 lysosomal membrane; GO:0031902 late endosome membrane