IRGM antibody

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Rabbit anti IRGM (Internal Region)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1887
Datasheet
IF P* WB
SDS
0.1 mg loader

Rabbit anti IRGM (Internal Region) antibody recognizes human Immunity-related GTPase family M protein (IRGM), also known as LRG47.

IRGM is a putative GTPase required for clearance of bacterial and protozoan infections. It funtions in the innate immune response, most likely through regulation of autophagy.

IRGM may also function in the regulation of proinflammatory cytokine production and in macrophage adhesion and motility.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human IRGM was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a 14 aa sequence from near the centre of human IRGM.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
A1A4Y4
Q6AYC2
Q60766
Entrez Gene
IRGM
Irgm1
Irgm
GO Terms
GO:0005886 plasma membrane
GO:0000139 Golgi membrane
GO:0000421 autophagic vacuole membrane
GO:0005525 GTP binding
GO:0006914 autophagy
GO:0006954 inflammatory response
GO:0016817 hydrolase activity, acting on acid anhydrides
GO:0030670 phagocytic vesicle membrane
GO:0042995 cell projection
GO:0045087 innate immune response
GO:0005783 endoplasmic reticulum
GO:0005765 lysosomal membrane
GO:0031902 late endosome membrane
Regulatory
For research purposes only

Applications of IRGM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 20ug/ml
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 1 2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human brain
Western Blotting
AHP1887 detects a band of approximately 32 kDa in human brain cell lysate.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Histar Detection System STAR3000A C P 50 Tests