IRF3 antibody

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Rabbit anti IRF3

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VPA00756 Datasheet	WB SDS	100 µl	Log in		Get Quote

Rabbit anti Human IRF3 antibody recognizes IRF3, also known as interferon regulatory factor 3.

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene (provided by RefSeq, Nov 2011).

Rabbit anti Human IRF3 antibody detects a band of 45-55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti IRF3 antibody recognizes a band of approximately 45-55 kDa in HeLa cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Rabbit polyclonal antibody purified by affinity chromatography on immunogen

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
2% Sucrose

Immunogen

KLH conjugated synthetic peptide corresponding to a portion of the C terminal region of human IRF3

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Guarantee: 12 months from date of despatch

More Information

UniProt: Q14653
Entrez Gene: IRF3
GO Terms: GO:0005886 plasma membrane; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0003677 DNA binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0003702 RNA polymerase II transcription factor activity; GO:0003712 transcription cofactor activity; GO:0009615 response to virus; GO:0005829 cytosol; GO:0005654 nucleoplasm; GO:0006355 regulation of transcription, DNA-dependent; GO:0006366 transcription from RNA polymerase II promoter; GO:0008063 Toll signaling pathway; GO:0010008 endosome membrane; GO:0032480 negative regulation of type I interferon production; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0044419 interspecies interaction between organisms; GO:0045087 innate immune response; GO:0060333 interferon-gamma-mediated signaling pathway; GO:0060337 type I interferon-mediated signaling pathway
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of IRF3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in		Get Quote

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