IL-21 antibody
Rabbit anti Human interleukin-21 antibody recognizes human Interleukin-21 (IL-21), also known as Za11. IL-21 is a cytokine with immunoregulatory activity. IL-21 stimulates B- and T-cell proliferation and increases the activity of NK cells and disease-specific cytotoxic T-cells. It might also inhibit dendritic cell activation and maturation during the T-cell mediated immune response.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human IL-21 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide corresponding to 15 amino acids from near the centre of human IL-21 precursor.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9HBE4
- Entrez Gene
- IL21
- GO Terms
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0005134 interleukin-2 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0032740 positive regulation of interleukin-17 production
- GO:0034105 positive regulation of tissue remodeling
- GO:0042102 positive regulation of T cell proliferation
- GO:0045078 positive regulation of interferon-gamma biosynthetic process
- GO:0048469 cell maturation
- GO:0050729 positive regulation of inflammatory response
- Regulatory
- For research purposes only
Applications of IL-21 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 5ug/ml | ||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human spleen
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for IL-21 antibody
-
Tripodo, C. et al. (2010) Mast Cells and Th17 Cells Contribute to the Lymphoma-Associated Pro-Inflammatory Microenvironment of Angioimmunoblastic T-Cell Lymphoma.
Am J Pathol. 177: 792-802. -
Coit, P. et al. (2016) DNA methylation analysis of the temporal artery microenvironment in giant cell arteritis.
Ann Rheum Dis. 75 (6): 1196-202.
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