IgG antibody
Goat F(ab')2 anti Human IgG:RPE
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Goat F(ab′)2 anti Human IgG antibody recognizes human IgG and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses. Goat F(ab′)2 anti Human IgG antibody does not cross-react with Goat IgG F(c) or mouse, rat, bovine and horse serum proteins, as determined by immunoelectrophoresis. Goat F(ab′)2 anti Human IgG antibody has been used for the detection of the antibody reponse to an endogenous retrovirus GAG matrix in patients with rheumatoid arthritis by ELISA (Nelson et al. 2014). |
- Target Species
- Human
- Product Form
- F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Antiserum Preparation
- Antisera to human IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Immunogen
- Human IgG.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for IgG antibody
-
Nelson, P.N. et al. (2014) Rheumatoid Arthritis is Associated with IgG Antibodies to Human Endogenous Retrovirus Gag Matrix: A Potential Pathogenic Mechanism of Disease?
J Rheumatol. 41: 1952-60. -
Trela, M. et al. (2019) Citrullination facilitates cross-reactivity of rheumatoid factor with non-IgG1 Fc epitopes in rheumatoid arthritis.
Sci Rep. 9 (1): 12068.
- RRID
- AB_322978
- UniProt
- P01857
- P01834
- P01860
- P01859
- P01861
- P0CG04
- Entrez Gene
- IGKC
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- IGLV2-14
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
STAR97PE
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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