HLA-DQA2 antibody

100% Secure


Rabbit anti HLA-DQA2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00443
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human HLA-DQA2 antibody recognizes the HLA class II histocompatibility antigen DQ alpha 2 chain, also known as DX alpha chain, HLA class II histocompatibility antigen, DQ (6) alpha chain or MHC class II DQA2.

HLA-DQA2 belongs to the HLA class II alpha chain family. The HLA-DQA2 gene encoded protein forms a heterodimer with a class II beta chain. It is located in intracellular vesicles and plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (B-cells, dendritic cells, macrophages) and are used to present antigenic peptides on the cell surface to be recognized by CD4 T-cells (provided by RefSeq, Jun 2010).

Rabbit anti Human HLA-DQA2 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti HLA-DQA2 detects a band of approximately 28 kDa in Raji cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 39-66 of human HLA-DQA2

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P01906 Related reagents
Entrez Gene
HLA-DQA2 Related reagents
GO Terms
GO:0002504 antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
GO:0042613 MHC class II protein complex
GO:0032395 MHC class II receptor activity
GO:0005887 integral to plasma membrane
GO:0005765 lysosomal membrane
GO:0005789 endoplasmic reticulum membrane
GO:0005794 Golgi apparatus
GO:0006955 immune response
GO:0010008 endosome membrane
GO:0031295 T cell costimulation
GO:0050852 T cell receptor signaling pathway
GO:0060333 interferon-gamma-mediated signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of HLA-DQA2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

Western Blotting without Trial and Error: Use PrecisionAb Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.