High Mobility Group Protein B1 antibody

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Rabbit anti High Mobility Group Protein B1

Rabbit anti-human high mobility group protein B1 antibody recognizes high mobility group protein B1, also known as Amphoterin, HMG-1, sulfoglucuronyl carbohydrate binding protein or high-mobility group box 1.

High Mobility Group Protein B1 Antibody with Control Lysate

Rabbit anti-human high mobility group protein B1 antibody recognizes high mobility group protein B1, also known as Amphoterin, HMG-1, sulfoglucuronyl carbohydrate binding protein or high-mobility group box 1.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00456 WB 100 µl
VPA00456KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human high mobility group protein B1 antibody recognizes high mobility group protein B1, also known as amphoterin, HMG-1 or Sulfoglucuronyl carbohydrate binding protein,

Rabbit anti Human high mobility group protein B1 antibody detects bands of 25 and 29 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose
Immunogen
Synthetic peptide directed towards the N terminal region of human high mobility group protein B1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P09429 Related reagents
Entrez Gene
HMGB1 Related reagents
GO Terms
GO:0000793 condensed chromosome
GO:0001773 myeloid dendritic cell activation
GO:0002407 dendritic cell chemotaxis
GO:0002437 inflammatory response to antigenic stimulus
GO:0003684 damaged DNA binding
GO:0003690 double-stranded DNA binding
GO:0003697 single-stranded DNA binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0006265 DNA topological change
GO:0006288 base-excision repair, DNA ligation
GO:0006309 DNA fragmentation involved in apoptotic nuclear change
GO:0008301 DNA bending activity
GO:0009986 cell surface
GO:0017055 negative regulation of RNA polymerase II transcriptional preinitiation complex assembly
GO:0031175 neuron projection development
GO:0033151 V(D)J recombination
GO:0042056 chemoattractant activity
GO:0043065 positive regulation of apoptosis
GO:0043280 positive regulation of caspase activity
GO:0043388 positive regulation of DNA binding
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050786 RAGE receptor binding
GO:0070491 repressing transcription factor binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of High Mobility Group Protein B1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti High mobility group protein B1 detects bands of approximately 25 & 29 kDa in C6 cell lysates
Western Blotting
Anti high mobility group protein B1 detects bands of approximately 25 & 29 kDa in C6 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB