Heat Shock Protein 90 Beta antibody

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Rabbit anti Heat Shock Protein 90 Beta

Heat shock protein 90 beta antibody recognizes the heat shock protein 90 beta, also known as HSP90-beta, heat shock 84 kDa or heat shock 90kD protein 1 beta.

Heat Shock Protein 90 Beta Antibody with Control Lysate

Heat shock protein 90 beta antibody recognizes the heat shock protein 90 beta, also known as HSP90-beta, heat shock 84 kDa or heat shock 90kD protein 1 beta.

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00800	WB	100 µl
VPA00800KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Rabbit anti Human heat shock protein 90 beta antibody recognizes the heat shock protein 90 beta, also known as HSP90-beta, heat shock 84 kDa or heat shock 90kD protein 1 beta.

HSP90AB1 encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. HSP90AB1 encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes (provided by RefSeq, Dec 2012).

Rabbit anti Human heat shock protein 90 beta antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti heat shock protein 90 beta antibody recognizes a band of approximately 90 kDa in HEK293 cell lysates
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Preparation: 20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose
Immunogen: Synthetic peptide directed towards the C terminal region of human heat shock protein 90 beta
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: P08238 Related reagents
Entrez Gene: HSP90AB1 Related reagents
GO Terms: GO:0051082 unfolded protein binding; GO:0006457 protein folding; GO:0006986 response to unfolded protein; GO:0005829 cytosol; GO:0005524 ATP binding; GO:0007411 axon guidance; GO:0030235 nitric-oxide synthase regulator activity; GO:0030911 TPR domain binding; GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process; GO:0042470 melanosome; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0060334 regulation of interferon-gamma-mediated signaling pathway; GO:0060338 regulation of type I interferon-mediated signaling pathway
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of Heat Shock Protein 90 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB
Goat anti Rabbit IgG (H/L):HRP	STAR208P	2 ml	WB

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