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Heat Shock Protein 60 antibody

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Anti Heat Shock Protein 60 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti Heat Shock Protein 60 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with heat shock protein 60 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to heat shock protein 60 (molecular weight 60 kDa)

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Rabbit anti Heat Shock Protein 60

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Heat Shock Protein 60

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VPA00897
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Rabbit anti Human heat shock protein 60 antibody recognizes heat shock protein 60 also known as 60 kDa chaperonin, HSPD1, HSP60, GROEL and mitochondrial matrix protein P1.

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13 (provided by RefSeq, Jun 2010).

Rabbit anti Human heat shock protein 60 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti heat shock protein 60 antibody recognizes a band of approximately 60 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human heat shock protein 60
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
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Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P10809
Entrez Gene
HSPD1
GO Terms
GO:0051082 unfolded protein binding
GO:0001530 lipopolysaccharide binding
GO:0002039 p53 binding
GO:0002368 B cell cytokine production
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002842 positive regulation of T cell mediated immune response to tumor cell
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0051087 chaperone binding
GO:0006986 response to unfolded protein
GO:0042026 protein refolding
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0005615 extracellular space
GO:0005743 mitochondrial inner membrane
GO:0005759 mitochondrial matrix
GO:0005769 early endosome
GO:0030141 stored secretory granule
GO:0005905 coated pit
GO:0006458 'de novo' protein folding
GO:0051131 chaperone-mediated protein complex assembly
GO:0006919 activation of caspase activity
GO:0016887 ATPase activity
GO:0009986 cell surface
GO:0050821 protein stabilization
GO:0030135 coated vesicle
GO:0032727 positive regulation of interferon-alpha production
GO:0032729 positive regulation of interferon-gamma production
GO:0032733 positive regulation of interleukin-10 production
GO:0032735 positive regulation of interleukin-12 production
GO:0032755 positive regulation of interleukin-6 production
GO:0042100 B cell proliferation
GO:0042110 T cell activation
GO:0043032 positive regulation of macrophage activation
GO:0043065 positive regulation of apoptosis
GO:0043066 negative regulation of apoptosis
GO:0043498 cell surface binding
GO:0044419 interspecies interaction between organisms
GO:0046696 lipopolysaccharide receptor complex
GO:0048291 isotype switching to IgG isotypes
GO:0050870 positive regulation of T cell activation
GO:0051604 protein maturation

VPA00897

180704

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