Hadha antibody
Rabbit anti Hadha
Rabbit anti-human HADHA antibody recognizes the trifunctional enzyme subunit alpha, mitochondrial, also known as 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit, 3-oxoacyl-CoA thiolase, or 78 kDa gastrin-binding protein.
Hadha Antibody with Control Lysate
Rabbit anti-human HADHA antibody recognizes the trifunctional enzyme subunit alpha, mitochondrial, also known as 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit, 3-oxoacyl-CoA thiolase, or 78 kDa gastrin-binding protein.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00446 | WB | 100 µl | ||||
| VPA00446KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human HADHA antibody recognizes HADHA, also known as 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit, 3-oxoacyl-CoA thiolase, 78 kDa gastrin-binding protein or long-chain-3-hydroxyacyl-CoA dehydrogenase.
HADHA gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in HADHA gene result in trifunctional protein deficiency (MTPD). The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation (provided by RefSeq, Jul 2008).
Rabbit anti Human HADHA antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
HADHA gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in HADHA gene result in trifunctional protein deficiency (MTPD). The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation (provided by RefSeq, Jul 2008).
Rabbit anti Human HADHA antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide corresponding to amino acid 737-763 of human HADHA
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P40939 Related reagents
- Entrez Gene
- HADHA Related reagents
- GO Terms
- GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity
- GO:0003985 acetyl-CoA C-acetyltransferase activity
- GO:0004300 enoyl-CoA hydratase activity
- GO:0005730 nucleolus
- GO:0006635 fatty acid beta-oxidation
- GO:0016507 fatty acid beta-oxidation multienzyme complex
- GO:0016509 long-chain-3-hydroxyacyl-CoA dehydrogenase activity
- GO:0042645 mitochondrial nucleoid
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Hadha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti HADHA detects a band of approximately 78 kDa in HepG2 cell lysates
- Western Blotting
- Anti HADHA detects a band of approximately 78 kDa in HepG2 cell lysates
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
