Hadha antibody

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Anti Hadha Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti Hadha Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti Hadha Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti Hadha Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with HADHA antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to HADHA (molecular weight 78 kDa).

Anti Hadha Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of HADHA knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with (A) Rabbit anti HADHA antibody (**VPA00446**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 60 second and (B) 30 second exposure.

Anti Hadha Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of HADHA from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Rabbit anti Hadha

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Hadha

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00446 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human HADHA antibody recognizes HADHA, also known as 3-ketoacyl-Coenzyme A (CoA) thiolase, alpha subunit, 3-oxoacyl-CoA thiolase, 78 kDa gastrin-binding protein or long-chain-3-hydroxyacyl-CoA dehydrogenase.

HADHA gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in HADHA gene result in trifunctional protein deficiency (MTPD). The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation (provided by RefSeq, Jul 2008).

Rabbit anti Human HADHA antibody detects a band of 78 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti HADHA detects a band of approximately 78 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH conjugated synthetic peptide corresponding to amino acid 737-763 of human HADHA
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P40939
Entrez Gene: HADHA
GO Terms: GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity; GO:0003985 acetyl-CoA C-acetyltransferase activity; GO:0004300 enoyl-CoA hydratase activity; GO:0005730 nucleolus; GO:0006635 fatty acid beta-oxidation; GO:0016507 fatty acid beta-oxidation multienzyme complex; GO:0016509 long-chain-3-hydroxyacyl-CoA dehydrogenase activity; GO:0042645 mitochondrial nucleoid