Glucagon antibody
Goat anti Human glucagon antibody (4660-1140) used for the demonstration of glucagon expressing cells in murine embryonic tissue sections by immunofluorescence.
Image caption:
Spatiotemporal expression of Sel1l during development of the mouse pancreas. Pancreatic sections from timed Sel1l+/βgeo embryos were co-immunostained with antibodies against βGal (red) and various pancreatic markers (green) as indicated on the left side of each panel. (B and H) Magnified views of the boxed area in A and G, respectively. (A-D) βGal expression at E12.5. βGal expression begins in a small number of cells within the core pancreatic epithelium at E12.5 (A). At this stage, there is no co-expression of βGal and SOX9 (B); however, βGal is co-expressed with either glucagon (C) or insulin (D). (E-F) βGal expression at E14.5. βGal is differentially expressed in the PDX1+ cells (E). While high βGal signal was detected in the epithelial branches (arrows), no or very low Gal signal was detected in the core "duct-like" epithelium (arrowhead). These core epithelial cells correspond to the subset of PDX+ cells that express SOX9 (F). (G-J) βGal expression at E15.5. βGal expression increases markedly in the core pancreatic epithelium (G and H, asterisks). βGal co-localizes with either glucagon (I, arrows) or insulin (J, arrows). (K-L) βGal expression at postnatal day 1. βGal is expressed in both islet and acinar tissues (K, asterisk and arrow, respectively). βGal co-localizes with PDX1 in the islet tissue (L). Scale bars: (A, G, I and J) 50 μm; (B-F, K-L) 100 μm.
From: Li S, Francisco AB, Munroe RJ, Schimenti JC, Long Q. SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 2010 Feb 19;10:19.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Goat anti Human glucagon antibody (4660-1140) used for the demonstration of glucagon expressing cells in murine embryonic tissue sections by immunofluorescence.
Image caption:
Inhibited endocrine and exocrine cell differentiation in Sel1lβgeo/βgeo embryos. (A-F) Immunohistological analysis of the developing pancreas of Sel1l+/+ and Sel1lβgeo/βgeo embryos at E11.5 (A-B) and E15.5 (C-F); the genotypes of the pancreatic sections are indicated as "+/+" and "-/-", respectively. The following antibodies were used: Pdx1 (A-D, green), glucagon (A-D, red), insulin (E-F, red) and amylase (E-F, green). (A-B) At E11.5, Glu+ cells were detected in the pancreatic epithelium of wild-type and Sel1lβgeo/βgeo embryos in equal numbers. (C-D) At E15.5, the number of glucagon+ cells was reduced in Sel1lβgeo/βgeo embryos as compared to wild-type control. (E-F) No amylase+ cells were detected and the number of insulin+ cells was significantly reduced in Sel1lβgeo/βgeo embryos. (G-I) Statistical analyses of the numbers of glucagon+ and insulin+ cells in wild-type and Sel1lβgeo/βgeo embryos. Data were from three wild-type and three Sel1lβgeo/βgeo embryos. At E11.5, no significant difference in the number of glucagon+ cells in wild-type and Sel1lβgeo/βgeo embryos was detected (G). The number of glucagon+ and insulin+ cells was markedly lower in Sel1lβgeo/βgeo embryos than in wild-type control embryos (H and I, respectively). Scale bar: 100 μm.
From: Li S, Francisco AB, Munroe RJ, Schimenti JC, Long Q. SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 2010 Feb 19;10:19.
Goat anti Glucagon antibody recognizes the pancreatic polypeptide hormone glucagon, whose actions are antagonistic to insulin. Glucagon is produced by A cells within the Islets of Langerhans in the pancreas.
Tumors arising from glucagon producing cells are termed glucagonomas, and this antibody may be useful in identifying such tumours.
Tumors arising from glucagon producing cells are termed glucagonomas, and this antibody may be useful in identifying such tumours.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Porcine glucagon, amino acids 1-12, conjugated with bovine thyroglobulin.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01275
- Entrez Gene
- GCG
- GO Terms
- GO:0005886 plasma membrane
- GO:0005576 extracellular region
- GO:0005179 hormone activity
- GO:0005625 soluble fraction
- GO:0006112 energy reserve metabolic process
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0007631 feeding behavior
- GO:0050796 regulation of insulin secretion
- GO:0008283 cell proliferation
- GO:0071377 cellular response to glucagon stimulus
- Regulatory
- For research purposes only
Applications of Glucagon antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Product Specific References
References for Glucagon antibody
-
Li, S. et al. (2010) SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 10: 19.
