Glucagon antibody
Goat anti Human glucagon antibody (4660-1140) used for the demonstration of glucagon expressing cells in murine embryonic tissue sections by immunofluorescence.
Image caption:
Spatiotemporal expression of Sel1l during development of the mouse pancreas. Pancreatic sections from timed Sel1l+/βgeo embryos were co-immunostained with antibodies against βGal (red) and various pancreatic markers (green) as indicated on the left side of each panel. (B and H) Magnified views of the boxed area in A and G, respectively. (A-D) βGal expression at E12.5. βGal expression begins in a small number of cells within the core pancreatic epithelium at E12.5 (A). At this stage, there is no co-expression of βGal and SOX9 (B); however, βGal is co-expressed with either glucagon (C) or insulin (D). (E-F) βGal expression at E14.5. βGal is differentially expressed in the PDX1+ cells (E). While high βGal signal was detected in the epithelial branches (arrows), no or very low Gal signal was detected in the core "duct-like" epithelium (arrowhead). These core epithelial cells correspond to the subset of PDX+ cells that express SOX9 (F). (G-J) βGal expression at E15.5. βGal expression increases markedly in the core pancreatic epithelium (G and H, asterisks). βGal co-localizes with either glucagon (I, arrows) or insulin (J, arrows). (K-L) βGal expression at postnatal day 1. βGal is expressed in both islet and acinar tissues (K, asterisk and arrow, respectively). βGal co-localizes with PDX1 in the islet tissue (L). Scale bars: (A, G, I and J) 50 μm; (B-F, K-L) 100 μm.
From: Li S, Francisco AB, Munroe RJ, Schimenti JC, Long Q. SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 2010 Feb 19;10:19.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Goat anti Human glucagon antibody (4660-1140) used for the demonstration of glucagon expressing cells in murine embryonic tissue sections by immunofluorescence.
Image caption:
Inhibited endocrine and exocrine cell differentiation in Sel1lβgeo/βgeo embryos. (A-F) Immunohistological analysis of the developing pancreas of Sel1l+/+ and Sel1lβgeo/βgeo embryos at E11.5 (A-B) and E15.5 (C-F); the genotypes of the pancreatic sections are indicated as "+/+" and "-/-", respectively. The following antibodies were used: Pdx1 (A-D, green), glucagon (A-D, red), insulin (E-F, red) and amylase (E-F, green). (A-B) At E11.5, Glu+ cells were detected in the pancreatic epithelium of wild-type and Sel1lβgeo/βgeo embryos in equal numbers. (C-D) At E15.5, the number of glucagon+ cells was reduced in Sel1lβgeo/βgeo embryos as compared to wild-type control. (E-F) No amylase+ cells were detected and the number of insulin+ cells was significantly reduced in Sel1lβgeo/βgeo embryos. (G-I) Statistical analyses of the numbers of glucagon+ and insulin+ cells in wild-type and Sel1lβgeo/βgeo embryos. Data were from three wild-type and three Sel1lβgeo/βgeo embryos. At E11.5, no significant difference in the number of glucagon+ cells in wild-type and Sel1lβgeo/βgeo embryos was detected (G). The number of glucagon+ and insulin+ cells was markedly lower in Sel1lβgeo/βgeo embryos than in wild-type control embryos (H and I, respectively). Scale bar: 100 μm.
From: Li S, Francisco AB, Munroe RJ, Schimenti JC, Long Q. SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 2010 Feb 19;10:19.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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IF ResetGoat anti Glucagon (N-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Glucagon
- Region
- (N-TERMINAL)
Antibody Quality and 
Unrivaled 
| Goat anti Glucagon antibody recognizes the pancreatic polypeptide hormone glucagon, whose actions are antagonistic to insulin. Glucagon is produced by A cells within the Islets of Langerhans in the pancreas. Tumors arising from glucagon producing cells are termed glucagonomas, and this antibody may be useful in identifying such tumours. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Porcine glucagon, amino acids 1-12, conjugated with bovine thyroglobulin.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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References for Glucagon antibody
-
Li, S. et al. (2010) SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells.
BMC Dev Biol. 10: 19.
- RRID
- AB_2107337
- UniProt
- P01275
- Entrez Gene
- GCG
- GO Terms
- GO:0005886 plasma membrane
- GO:0005576 extracellular region
- GO:0005179 hormone activity
- GO:0005625 soluble fraction
- GO:0006112 energy reserve metabolic process
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0007631 feeding behavior
- GO:0050796 regulation of insulin secretion
- GO:0008283 cell proliferation
- View More GO Terms
- GO:0071377 cellular response to glucagon stimulus
View more products with GLUCAGON specificity
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