GCLC antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti GCLC Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with GCLC antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to GCLC (molecular weight 75 kDa)

Filter by Application:

Rabbit anti GCLC

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: GCLC

Quick Links:

Secondary Antibodies
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00695 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
	Log in

Rabbit anti Human GCLC antibody recognizes glutamate-cysteine ligase catalytic subunit, also known as GCS heavy chain, gamma-ECS or gamma-glutamylcysteine synthetase.

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction (provided by RefSeq, Oct 2010).

Rabbit anti Human GCLC antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Rabbit anti GCLC detects a band of approximately 75 kDa in HeLa cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide
2% Sucrose
Immunogen: Synthetic peptide encompassing part of the middle region of human GCLC
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P48506
Entrez Gene: GCLC
GO Terms: GO:0000287 magnesium ion binding; GO:0004357 glutamate-cysteine ligase activity; GO:0005829 cytosol; GO:0005524 ATP binding; GO:0045454 cell redox homeostasis; GO:0006534 cysteine metabolic process; GO:0006536 glutamate metabolic process; GO:0006750 glutathione biosynthetic process; GO:0006805 xenobiotic metabolic process; View More GO Terms; GO:0006916 anti-apoptosis; GO:0006979 response to oxidative stress; GO:0016595 glutamate binding; GO:0009408 response to heat; GO:0009725 response to hormone stimulus; GO:0016481 negative regulation of transcription; GO:0043531 ADP binding; GO:0050662 coenzyme binding; GO:0050880 regulation of blood vessel size