GCLC antibody

Rabbit anti GCLC

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
GCLC

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VPA00695
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Rabbit anti Human GCLC antibody recognizes glutamate-cysteine ligase catalytic subunit, also known as GCS heavy chain, gamma-ECS or gamma-glutamylcysteine synthetase.

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction (provided by RefSeq, Oct 2010).

Rabbit anti Human GCLC antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti GCLC detects a band of approximately 75 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the middle region of human GCLC
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P48506
Entrez Gene
GCLC
GO Terms
GO:0000287 magnesium ion binding
GO:0004357 glutamate-cysteine ligase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0045454 cell redox homeostasis
GO:0006534 cysteine metabolic process
GO:0006536 glutamate metabolic process
GO:0006750 glutathione biosynthetic process
GO:0006805 xenobiotic metabolic process
GO:0006916 anti-apoptosis
GO:0006979 response to oxidative stress
GO:0016595 glutamate binding
GO:0009408 response to heat
GO:0009725 response to hormone stimulus
GO:0016481 negative regulation of transcription
GO:0043531 ADP binding
GO:0050662 coenzyme binding
GO:0050880 regulation of blood vessel size
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products