Furin antibody
Goat anti Human furin antibody recognizes an epitope corresponding to aa553-565 of human Furin, otherwise known as PACE (Paired basic Amino acid Cleaving Enzyme). Furin is a calcium-dependent serine endoprotease and proprotein convertase, which plays a crucial role in the processing of latent precursor proteins into their biologically active forms, such as during the Notch signaling pathway.
The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.
Furin is a critical enzyme during the S1 cleavage of pre-Notch proteins within the golgi apparatus, to convert the nascent form into the mature heterodimeric Notch receptor and the non-covalently associated intracellular domain. These are then transported to the cell surface, in preparation for ligand-receptor binding and further S2 and S3 cleavage, resulting in the release and translocation of the intracellular domain to the nucleus.
The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.
Furin is a critical enzyme during the S1 cleavage of pre-Notch proteins within the golgi apparatus, to convert the nascent form into the mature heterodimeric Notch receptor and the non-covalently associated intracellular domain. These are then transported to the cell surface, in preparation for ligand-receptor binding and further S2 and S3 cleavage, resulting in the release and translocation of the intracellular domain to the nucleus.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence Dog Expected from Sequence Pig Expected from Sequence Bovine Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human Furin was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence C-NTSEANNYGTLTK from the internal region of Furin (NP_002560.1).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P09958 Related reagents
- Entrez Gene
- FURIN Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0000139 Golgi membrane
- GO:0002020 protease binding
- GO:0004252 serine-type endopeptidase activity
- GO:0016021 integral to membrane
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0048406 nerve growth factor binding
- GO:0046872 metal ion binding
- GO:0005796 Golgi lumen
- GO:0005802 trans-Golgi network
- GO:0006465 signal peptide processing
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0007219 Notch signaling pathway
- GO:0008283 cell proliferation
- GO:0009986 cell surface
- GO:0017187 peptidyl-glutamic acid carboxylation
- GO:0019067 viral assembly, maturation, egress, and release
- GO:0030140 trans-Golgi network transport vesicle
- GO:0032455 nerve growth factor processing
- GO:0032804 negative regulation of low-density lipoprotein particle receptor catabolic process
- GO:0032902 nerve growth factor production
- GO:0032911 negative regulation of transforming growth factor-beta1 production
- GO:0032940 secretion by cell
- GO:0042277 peptide binding
- GO:0043043 peptide biosynthetic process
- GO:0043687 post-translational protein modification
- GO:0045121 membrane raft
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- Regulatory
- For research purposes only
Applications of Furin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/8000 | ||
| Immunohistology - Paraffin 1 | 2.0 | 4.0ug/ml | |
| Western Blotting |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human liver
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P |
Product Specific References
Further Reading
-
Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
Nat Rev Mol Cell Biol. 7 (9): 678-89.
