Furin antibody

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Goat anti Human Furin (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2262
Datasheet
E WB
MSDS
0.1 mg

Goat anti Human furin antibody recognizes an epitope within the C-Terminal (CT) region of furin, otherwise known as PACE (Paired basic Amino acid Cleaving Enzyme). Furin is a calcium-dependent serine endoprotease and proprotein convertase, which plays a crucial role in the processing of latent precursor proteins into their biologically active forms, such as during the Notch signaling pathway.

The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.

Furin is a critical enzyme during the S1 cleavage of pre-Notch proteins within the golgi apparatus, to convert the nascent form into the mature heterodimeric Notch receptor and the non-covalently associated intracellular domain. These are then transported to the cell surface, in preparation for ligand-receptor binding and further S2 and S3 cleavage, resulting in the release and translocation of the intracellular domain to the nucleus.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Dog Expected from Sequence
Pig Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human Furin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-EDEGRGERTAFIKDQ, from within the C-Terminal region of Furin (NP_002560.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P09958 Related reagents
Entrez Gene
FURIN Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000139 Golgi membrane
GO:0002020 protease binding
GO:0004252 serine-type endopeptidase activity
GO:0016021 integral to membrane
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0048406 nerve growth factor binding
GO:0046872 metal ion binding
GO:0005796 Golgi lumen
GO:0005802 trans-Golgi network
GO:0006465 signal peptide processing
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007219 Notch signaling pathway
GO:0008283 cell proliferation
GO:0009986 cell surface
GO:0017187 peptidyl-glutamic acid carboxylation
GO:0019067 viral assembly, maturation, egress, and release
GO:0030140 trans-Golgi network transport vesicle
GO:0032455 nerve growth factor processing
GO:0032804 negative regulation of low-density lipoprotein particle receptor catabolic process
GO:0032902 nerve growth factor production
GO:0032911 negative regulation of transforming growth factor-beta1 production
GO:0032940 secretion by cell
GO:0042277 peptide binding
GO:0043043 peptide biosynthetic process
GO:0043687 post-translational protein modification
GO:0045121 membrane raft
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
Regulatory
For research purposes only

Applications of Furin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/128000
Western Blotting 0.01 0.03ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2262 detects a band of approximately 110kDa in HEK293 lysate transiently expressing Human Furin tagged with myc and DYKDDDDK.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

Further Reading

  1. Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
    Nat Rev Mol Cell Biol. 7 (9): 678-89.