The Notch signaling pathway is an evolutionarily conserved pathway in multicellular organisms, vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell hematopoiesis, thymic T cell development, and both tumor progression and suppression.
Furin is a critical enzyme during the S1 cleavage of pre-Notch proteins within the golgi apparatus, to convert the nascent form into the mature heterodimeric Notch receptor and the non-covalently associated intracellular domain. These are then transported to the cell surface, in preparation for ligand-receptor binding and further S2 and S3 cleavage, resulting in the release and translocation of the intracellular domain to the nucleus.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence Dog Expected from Sequence Pig Expected from Sequence Bovine Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human Furin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
- Synthetic peptide sequence C-EDEGRGERTAFIKDQ, from within the C-Terminal region of Furin (NP_002560.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0000139 Golgi membrane
- GO:0002020 protease binding
- GO:0004252 serine-type endopeptidase activity
- GO:0016021 integral to membrane
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0048406 nerve growth factor binding
- GO:0046872 metal ion binding
- GO:0005796 Golgi lumen
- GO:0005802 trans-Golgi network
- GO:0006465 signal peptide processing
- GO:0007179 transforming growth factor beta receptor signaling pathway
- GO:0007219 Notch signaling pathway
- GO:0008283 cell proliferation
- GO:0009986 cell surface
- GO:0017187 peptidyl-glutamic acid carboxylation
- GO:0019067 viral assembly, maturation, egress, and release
- GO:0030140 trans-Golgi network transport vesicle
- GO:0032455 nerve growth factor processing
- GO:0032804 negative regulation of low-density lipoprotein particle receptor catabolic process
- GO:0032902 nerve growth factor production
- GO:0032911 negative regulation of transforming growth factor-beta1 production
- GO:0032940 secretion by cell
- GO:0042277 peptide binding
- GO:0043043 peptide biosynthetic process
- GO:0043687 post-translational protein modification
- GO:0045121 membrane raft
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- For research purposes only
Applications of Furin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
- Western Blotting
- AHP2262 detects a band of approximately 110kDa in HEK293 lysate transiently expressing Human Furin tagged with myc and DYKDDDDK.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Rabbit anti Goat IgG (Fc):FITC||STAR122F||F||1 mg|
|Rabbit anti Goat IgG (Fc):HRP||STAR122P||C E WB||1 mg|
Product Specific References
Bray, S.J. (2006) Notch signalling: a simple pathway becomes complex.
Nat Rev Mol Cell Biol. 7 (9): 678-89.
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