FTCD antibody

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Goat anti Human FTCD (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1063 E WB 0.1 mg
Goat anti Human FTCD antibody detects an epitope within the N-terminal (NT) region of formiminotransferase cyclodeaminase (FTCD). FTCD is a homo-octameric bifunctional enzyme which plays important roles in coupling histidine catabolism with folate metabolism, and integrating the Golgi complex with the vimentin intermediate filament cytoskeleton.

FTCD is present in all mammalian cells either free in the cytosol or tightly bound to the Golgi complex, and is most abundant in the liver. FTCD is linked to two human diseases, autoimmune hepatitis and glutamate formiminotransferase deficiency (Mao et al. 2004).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to FTCD were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence SQLVECVPNFSEGKNQ from the N-terminus of FTCD.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O95954 Related reagents
Entrez Gene
FTCD Related reagents
GO Terms
GO:0005829 cytosol
GO:0005542 folic acid binding
GO:0005794 Golgi apparatus
GO:0005814 centriole
GO:0006548 histidine catabolic process
GO:0006760 folic acid-containing compound metabolic process
GO:0030409 glutamate formimidoyltransferase activity
GO:0030412 formimidoyltetrahydrofolate cyclodeaminase activity
Regulatory
For research purposes only

Applications of FTCD antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/64000
Western Blotting 0.03 0.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1063 detects a band of approximately 55-60kDa in human liver lysate.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Product Specific References

References for FTCD antibody

  1. Mao, Y. et al. (2004) Structure of the bifunctional and Golgi-associated formiminotransferase cyclodeaminase octamer.
    EMBO J. 23 (15): 2963-71.
  2. Renous, R. et al. (2004) Characterization of the antigenicity of the formiminotransferase-cyclodeaminase in type 2 autoimmune hepatitis.
    Exp Cell Res. 292 (2): 332-41.