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FOXA1 antibody

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Anti FOXA1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti FOXA1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti FOXA1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti FOXA1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with FOXA1 (VPA00720) antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 40 second exposure. Arrow points to FOXA1 (molecular weight 50 kDa).
Anti FOXA1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of FOXA1 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Rabbit anti FOXA1 antibody (VPA00720) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 600 second and (B) 30 second exposure.
Anti FOXA1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of FOXA1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Rabbit anti FOXA1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
FOXA1

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Product Code Applications Pack Size List Price Your Price Qty
VPA00720
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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Rabbit anti Human FOXA1 antibody recognizes the hepatocyte nuclear factor 3-alpha, also known as HNF-3-alpha, HNF-3A, TCF-3A or transcription factor 3A.

The FOXA1 gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver (provided by RefSeq, Jul 2008).

Rabbit anti Human FOXA1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti FOXA1 detects a band of approximately 50 kDa in HepG2 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
<50% Glycerol
Immunogen
Carrier-protein conjugated synthetic peptide encompassing part of the C-terminal region of human FOXA1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P55317
Entrez Gene
FOXA1
GO Terms
GO:0003690 double-stranded DNA binding
GO:0003704 specific RNA polymerase II transcription factor activity
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005667 transcription factor complex
GO:0006338 chromatin remodeling
GO:0009790 embryo development
GO:0007389 pattern specification process
GO:0008134 transcription factor binding
GO:0008301 DNA bending activity
GO:0008634 negative regulation of survival gene product expression
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016566 specific transcriptional repressor activity
GO:0019904 protein domain specific binding
GO:0032355 response to estradiol stimulus
GO:0033148 positive regulation of estrogen receptor signaling pathway
GO:0042593 glucose homeostasis
GO:0045666 positive regulation of neuron differentiation
GO:0045931 positive regulation of mitotic cell cycle
GO:0048665 neuron fate specification
GO:0051091 positive regulation of transcription factor activity
GO:0060441 epithelial tube branching involved in lung morphogenesis
GO:0060487 lung epithelial cell differentiation
GO:0060528 secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development
GO:0060738 epithelial-mesenchymal signaling involved in prostate gland development
GO:0060740 prostate gland epithelium morphogenesis
GO:0060741 prostate gland stromal morphogenesis
GO:0060743 epithelial cell maturation involved in prostate gland development

VPA00720

161118

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