FHL1 antibody

Goat anti Human FHL1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
FHL1

Product Code Applications Pack Size List Price Your Price Qty
AHP2070
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human FHL1 antibody recognizes human Four and a half LIM domains protein 1, also known as FHL1, Skeletal muscle LIM-protein 1 or SLIM-1. FHL1 is a 323 amino acid ~30 kDa cytoplasmic protein containing multiple LIM domains. FHL1 is abundantly expressed by skeletal muscle and has been linked with several muscular dystrophy-like muscle disorders, including Scapuloperoneal syndrome (Quinzii et al. 2008), X-linked severe early-onset reducing body myopathy (Schlessl et al. 2011), and X-linked myopathy with postural muscle atrophy (Windpassinger et al. 2008).

Goat anti Human FHL1 antibody is expected to recognise FHL1 isoform 5 and isoform 2, the latter being implicated as a negative regulator of RBP J- mediated transcription (Liang et al. 2008).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Mouse
Rat Expected from Sequence
Bovine Expected from Sequence
Pig Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human FHL1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence CNKRFVFHNEQVY from the internal region of FHL1 (NP_001440.1; NP_001153171.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Immunohistology - Paraffin 1 2.0 2.5ug/ml
Western Blotting 0.05 0.3ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human testis.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

References for FHL1 antibody

  1. Emmanuele, V. et al. (2015) Fhl1 W122S causes loss of protein function and late-onset mild myopathy.
    Hum Mol Genet. 24 (3): 714-26.
  2. Can, T. et al. (2014) Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
    Proteome Sci. 12: 25.

Synonyms
SLIM1
RRID
AB_2104218
UniProt
Q13642
Entrez Gene
FHL1
View more products with FHL1 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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