FHL1 antibody
Goat anti Human FHL1 antibody is expected to recognise FHL1 isoform 5 and isoform 2, the latter being implicated as a negative regulator of RBP J- mediated transcription (Liang et al. 2008).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Expected from Sequence Mouse Rat Expected from Sequence Bovine Expected from Sequence Pig Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human FHL1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence CNKRFVFHNEQVY from the internal region of FHL1 (NP_001440.1; NP_001153171.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
Applications of FHL1 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/32000 | ||
Immunohistology - Paraffin 1 | 2.0 | 2.5ug/ml | |
Western Blotting | 0.05 | 0.3ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human testis.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for FHL1 antibody
-
Emmanuele, V. et al. (2015) Fhl1 W122S causes loss of protein function and late-onset mild myopathy.
Hum Mol Genet. 24 (3): 714-26. -
Can, T. et al. (2014) Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
Proteome Sci. 12: 25.
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