FHL1 antibody
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WB P ResetGoat anti Human FHL1
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- FHL1
Antibody Quality and Performance Guarantee
| Goat anti Human FHL1 antibody recognizes human Four and a half LIM domains protein 1, also known as FHL1, Skeletal muscle LIM-protein 1 or SLIM-1. FHL1 is a 323 amino acid ~30 kDa cytoplasmic protein containing multiple LIM domains. FHL1 is abundantly expressed by skeletal muscle and has been linked with several muscular dystrophy-like muscle disorders, including Scapuloperoneal syndrome (Quinzii et al. 2008), X-linked severe early-onset reducing body myopathy (Schlessl et al. 2011), and X-linked myopathy with postural muscle atrophy (Windpassinger et al. 2008). Goat anti Human FHL1 antibody is expected to recognise FHL1 isoform 5 and isoform 2, the latter being implicated as a negative regulator of RBP J- mediated transcription (Liang et al. 2008). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Expected from Sequence Mouse Rat Expected from Sequence Bovine Expected from Sequence Pig Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human FHL1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence CNKRFVFHNEQVY from the internal region of FHL1 (NP_001440.1; NP_001153171.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/32000 | |
| Immunohistology - Paraffin 1 | |
2.0 | 2.5ug/ml |
| Western Blotting | |
0.05 | 0.3ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human testis.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
References for FHL1 antibody
-
Emmanuele, V. et al. (2015) Fhl1 W122S causes loss of protein function and late-onset mild myopathy.
Hum Mol Genet. 24 (3): 714-26. -
Can, T. et al. (2014) Proteomic analysis of laser capture microscopy purified myotendinous junction regions from muscle sections.
Proteome Sci. 12: 25.
- Synonyms
- SLIM1
- RRID
- AB_2104218
- UniProt
- Q13642
- Entrez Gene
- FHL1
AHP2070
152660
161028
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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