ErbB3 antibody

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Goat anti ErbB3

ErbB3 antibody recognizes receptor tyrosine-protein kinase ErbB-3, also known as proto-oncogene-like protein c-ErbB-3 tyrosine kinase-type cell surface receptor HER3. The antibody has been extensively validated for western blotting using whole cell lysates.

ErbB3 Antibody with Control Lysate

ErbB3 antibody recognizes receptor tyrosine-protein kinase ErbB-3, also known as proto-oncogene-like protein c-ErbB-3 tyrosine kinase-type cell surface receptor HER3. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00003 WB 100 µl
VPA00003KT WB 2 Westerns
With Lysate 400µg
Goat anti Human ErbB3 antibody recognizes the human receptor tyrosine-protein kinase ErbB-3, also known as proto-oncogene-like protein c-ErbB-3 or tyrosine kinase-type cell surface receptor HER3. ErbB3 is a 1342 amino acid single pass type 1 membrane glycoprotein, a tyrosine protein kinase containing a single protein kinase domain, originally considered inactive (Citri et al. 2003). However, subsequent studies have demonstrated that the kinase domain is competent to bind ATP and retains sufficient activity for the catalysis of autophosphorylation (Shi et al. 2010). Multiple isoforms of ErbB3, generated by alternative splicing have been described (Katoh et al. 1993).

Goat anti Human Erb3 antibody is generated against a peptide located within the C-terminal region and is expected to recognize membrane bound isoforms 1,4 and 5 but not the short isoforms 2 and 3. ErbB3 is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases and has neuregulin binding avtivity (Carraway et al. 1994). Heterodimerization of ErbB3 with other EGFR family members leads to cell proliferation and/or differentiation (Carraway and Cantley 1994).

Amplification of this gene and/or over expression of its protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. In breast cancer it has been shown to dimerize with ErbB2 to drive tumour cell proliferation (Alimandi et al. 1995).

Goat anti Human ErbB3 antibody recognizes human ErbB3 as a 185 kDa band in a number of human cell line lysates tested. It also demonstrates cross reacts with rodent cell lines.

Product Details

Target Species
Human
Western Blotting
Anti ErbB3 antibody detects a band of approximately 185 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
Preparation
20μl Goat polyclonal antibody by purified ammonium sulfate precipitation followed by antigen affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-HSRLFPKANAQRT from the C-terminal region ErbB3 isoform 1
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P21860 Related reagents
Entrez Gene
ERBB3 Related reagents
GO Terms
GO:0004714 transmembrane receptor protein tyrosine kinase activity
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0019838 growth factor binding
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005615 extracellular space
GO:0006468 protein phosphorylation
GO:0007162 negative regulation of cell adhesion
GO:0007507 heart development
GO:0009968 negative regulation of signal transduction
GO:0014037 Schwann cell differentiation
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0016323 basolateral plasma membrane
GO:0021545 cranial nerve development
GO:0030296 protein tyrosine kinase activator activity
GO:0042060 wound healing
GO:0042127 regulation of cell proliferation
GO:0042803 protein homodimerization activity
GO:0043235 receptor complex
GO:0043524 negative regulation of neuron apoptosis
GO:0046982 protein heterodimerization activity
GO:0051048 negative regulation of secretion
GO:0051402 neuron apoptosis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of ErbB3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (H/L):HRP 5160-2504 2 mg C E WB
Rabbit anti Goat IgG (H/L):HRP 5160-2504 2 mg C E WB

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