IgG antibody
Rabbit anti Goat IgG (H/L) antibody (5160-2104) used for the secondary detection of Goat anti Adenovirus antibody by western blotting.
Image caption:
C1 and C4 Do Not Prevent Virus Attachment or Internalization.
Western blot for adenovirus and actin.
From: Bottermann M, Foss S, Caddy SL, Clift D, van Tienen LM, Vaysburd M, Cruickshank J, O'Connell K, Clark J, Mayes K, Higginson K, Lode HE, McAdam MB, Sandlie I, Andersen JT, James LC.
Complement C4 Prevents Viral Infection through Capsid Inactivation.
Cell Host Microbe. 2019 Apr 10;25(4):617-629.e7.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Goat IgG (H/L) antibody (5160-2104) used for the secondary detection of Goat anti Adenovirus antibody by western blotting.
Image caption:
C1 and C4 Prevent Adenoviral Protein VI Exposure and Endosomal Escape
(A) IP of Ad5 with 9C12 after Ad5 was incubated at the indicated temperatures for 30 min. WB: anti-adenovirus.
From: Bottermann M, Foss S, Caddy SL, Clift D, van Tienen LM, Vaysburd M, Cruickshank J, O'Connell K, Clark J, Mayes K, Higginson K, Lode HE, McAdam MB, Sandlie I, Andersen JT, James LC.
Complement C4 Prevents Viral Infection through Capsid Inactivation.
Cell Host Microbe. 2019 Apr 10;25(4):617-629.e7.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
WB ResetRabbit anti Goat IgG (H/L):HRP
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
| Rabbit anti Goat IgG (H/L) antibody recognizes caprine IgG and recognizes both heavy and light chains. In immunoelectrophoresis, a single precipitin arc was observed against anti-peroxidase, anti-rabbit serum, goat IgG and goat serum. |
- Target Species
- Goat
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
- Reconstitution
- Use 1 ml of sterile distilled water.
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Gentamicin sulphate 1% Bovine Serum Albumin - Immunogen
- Goat IgG, whole molecule
- Approx. Protein Concentrations
- 2.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/80,000 | 1/240,000 |
| Immunohistology - Frozen | |
1/1,000 | 1/5,000 |
| Western Blotting | |
1/5,000 | 1/25,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | AbGUARD® HRP Stabilizer Plus | ||||||
| TMB Signal+ | BUF054A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TMB Signal+ | ||||||
| TMB Core | BUF056A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core | ||||||
| TMB Core+ | BUF062A | E | 100 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TMB Core+ | ||||||
References for IgG antibody
-
Bottermann, M. et al. (2019) Complement C4 Prevents Viral Infection through Capsid Inactivation.
Cell Host Microbe. 25 (4): 617-629.e7.
Further Reading
-
Farr, A.G. & Nakane, P.K. (1981) Immunohistochemistry with enzyme labeled antibodies: a brief review.
J Immunol Methods. 47 (2): 129-44.
- RRID
- AB_619875
5160-2504
152947
157576
165591
165597
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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