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Delta-Like Protein 1 antibody

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Anti Delta-Like Protein 1 Antibody thumbnail image 1 Anti Delta-Like Protein 1 Antibody thumbnail image 2 Anti Delta-Like Protein 1 Antibody thumbnail image 3 Anti Delta-Like Protein 1 Antibody thumbnail image 4 Anti Delta-Like Protein 1 Antibody thumbnail image 5
Anti Delta-Like Protein 1 Antibody gallery image 1
Western blot analysis of rat heart cell lysate probed with Goat anti Delta-like protein 1 antibody (AHP2251).
Anti Delta-Like Protein 1 Antibody gallery image 2
Western blotting analysis of mouse heart lysate (35μg protein in RIPA buffer) probed with Goat anti Human Delta-like protein 1 antibody (AHP2251) at 0.3μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Anti Delta-Like Protein 1 Antibody gallery image 3
Western blot image of rat pancreas lysate (35μg protein per lane in RIPA buffer) probed with Goat anti Delta-like Protein 1 antibody (AHP2251) at a concentration of 3μgml-1 (A) in the presence of immunizing peptide (B). Detection is by chemiluminescence.
Anti Delta-Like Protein 1 Antibody gallery image 4
Immunofluorescence image of paraformaldehyde fixed, triton permeablized HeLa cells probed with Goat anti Delta-like Protein 1 antibody (AHP2251) for 1 hr at a concentration of 10μgml-1 followed by Alexa Fluor 488 conjugated secondary (green). Actin filaments are stained with phalloidin (red) and nuclei are stained with DAPI (blue). Unimmunized goat IgG is used as a negative control.
Anti Delta-Like Protein 1 Antibody gallery image 5
Flow cytometric analysis of paraformaldehyde fixed, triton permeablized HeLa cells stained with Goat anti Delta-like Protein 1 antibody at 10μgml-1 (AHP2251) followed by Alexa Fluor 488 secondary reagent. Unimmunized goat IgG is used as a negative control (black line).

Goat anti Delta-Like Protein 1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Delta-Like Protein 1

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Product Code Applications Pack Size List Price Your Price Qty
AHP2251
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SDS Safety Datasheet SDS
E WB 0.1 mg loader
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Goat anti Human Delta-like protein 1 antibody recognizes Delta-like protein 1 (DLL1), one of the five major ligands of the Notch signalling pathway, activated through the binding of specific ligands to the Notch receptors Notch 1-4.

The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression.

Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation.

DLL1 is widely expressed, and acts as a mediator of cell fate decisions during haematopoiesis, and may play a role in cell-to-cell communication in mammalian embryos. DLL1 plays an important role in B and T cell differentiation, in embryonic somite formation and patterning, and associates with the scaffolding protein MAGI1 at adherens junctions on neuronal processes. Signalling through DLL1 and Notch 2 has been implicated in the development of marginal zone B cells (MZB).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human DLL1 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-ATQRHLTVGEEWSQD from the internal region of DLL1 (NP_005609.3).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/16,000
Western Blotting 0.3 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2251detects a band of approximately 75kDa in mouse and rat heart cell lysates.

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Synonyms
DLL1
RRID
AB_10846103
UniProt
O00548
P97677
Q61483
Entrez Gene
DLL1
Dll1
Dll1
GO Terms
GO:0005509 calcium ion binding
GO:0001709 cell fate determination
GO:0005576 extracellular region
GO:0005887 integral to plasma membrane
GO:0005112 Notch binding
GO:0007154 cell communication
GO:0007219 Notch signaling pathway
GO:0007220 Notch receptor processing
GO:0030097 hemopoiesis
GO:0030155 regulation of cell adhesion
GO:0016021 integral to membrane
GO:0009912 auditory receptor cell fate commitment
GO:0005886 plasma membrane
GO:0001701 in utero embryonic development
GO:0001757 somite specification
GO:0007368 determination of left/right symmetry
GO:0007386 compartment pattern formation
GO:0031410 cytoplasmic vesicle
GO:0042472 inner ear morphogenesis
GO:0042475 odontogenesis of dentine-containing tooth
GO:0045608 negative regulation of auditory receptor cell differentiation

AHP2251

160208

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