Collagen VI antibody

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Rabbit anti Collagen VI

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2049 P* 50 µg
Rabbit anti Human collagen VI antibody detects mammalian collagen type VI, also known as COL6A, a secreted extracellular matrix protein involved in cellular adhesion.

Collagen type specific antibodies are dependent on non-denatured three-dimensional epitopes. Therefore, the reactivity and selectivity of this antibody will be significantly reduced if denatured and reducing conditions of SDS-PAGE and immunblotting are used.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Mammals Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Collagen IV was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
0.125M Sodium Borate, 0.075 Sodium Chloride, 0.005M EDTA
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
Collagen Type VI from Human and Bovine Placenta.
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P12109 Related reagents
Entrez Gene
COL6A1 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0005589 collagen type VI
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
Regulatory
For research purposes only

Applications of Collagen VI antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1 5 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human breast or lung

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
Histar Detection System STAR3000A 50 Tests C P
Histar Detection System STAR3000B 150 Tests C P
Histar Detection System STAR3000C 500 Tests C P