Collagen VI antibody

Rabbit anti Collagen VI

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Collagen VI

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AHP2049
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Rabbit anti Human collagen VI antibody detects mammalian collagen type VI, also known as COL6A, a secreted extracellular matrix protein involved in cellular adhesion.

Collagen type specific antibodies are dependent on non-denatured three-dimensional epitopes. Therefore, the reactivity and selectivity of this antibody will be significantly reduced if denatured and reducing conditions of SDS-PAGE and immunblotting are used.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Mammals Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to Collagen IV was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
0.02M Potassium Phosphate, 0.15M NaCl
Preservative Stabilisers
0.01% Sodium Azide (NaN3)
Immunogen
Collagen Type VI from Human and Bovine Placenta.
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1 5 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human breast or lung

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RRID
AB_2083109
UniProt
P12109
Entrez Gene
COL6A1
GO Terms
GO:0007155 cell adhesion
GO:0005589 collagen type VI
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
View more products with COLLAGEN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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