Collagen IV Alpha 5 antibody

Rabbit anti Collagen IV Alpha 5

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Collagen IV Alpha 5

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VPA00836
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Rabbit anti Human collagen IV alpha 5 antibody recognizes collagen IV alpha 5, also known as collagen alpha-5(IV) chain, ATS, COL4A5, ASLN and collagen of basement membrane alpha-5 chain.

COL4A5 encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in COL4A5 are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, COL4A5 is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for COL4A5 (provided by RefSeq, Aug 2010).

Rabbit anti Human collagen IV alpha 5 antibody detects a band of 161 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti collagen IV alpha 5 antibody recognizes a band of approximately 161 kDa in RD cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Recombinant protein of human collagen IV alpha 5
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P29400
Entrez Gene
COL4A5
GO Terms
GO:0005515 protein binding
GO:0005201 extracellular matrix structural constituent
GO:0005587 collagen type IV
GO:0007411 axon guidance
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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