Clusterin antibody

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Goat anti Human Clusterin (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2129
Datasheet
E WB
MSDS
0.1 mg

Goat anti Human clusterin antibody recognizes an epitope within the C-terminal (CT) region of human clusterin, otherwise known as Apolipoprotein J, a ubiquitously expressed glycoprotein and member of the clusterin family, implicated in apoptosis (programmed cell death), cell-cell adhesion, DNA repair, membrane recycling, spermatogenesis, and the inhibition of complement-mediated cell lysis.

Clusterin is a component of the SC5b-9 complex which plays a role in inhibiting the complement cascade, through the prevention of the binding of a C5b-C7 complex to the membrane of the target cell. Clusterin exists as both a pro-apoptotic form localised to the nucleus, and a pro-survival secreted form, and has been detected at elevated levels in Alzheimer’s disease, atherosclerosis, myocardial infarction, and various cancers, including bladder, prostate, and breast tumours.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human clusterin (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence C-EKALQEYRKKHREE from the C-Terminal region of clusterin (NP_001822.2; NP_976084.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P10909
Entrez Gene
CLU
GO Terms
GO:0001836 release of cytochrome c from mitochondria
GO:0002576 platelet degranulation
GO:0009615 response to virus
GO:0005739 mitochondrion
GO:0006629 lipid metabolic process
GO:0006958 complement activation, classical pathway
GO:0051787 misfolded protein binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0032463 negative regulation of protein homooligomerization
GO:0034366 spherical high-density lipoprotein particle
GO:0043691 reverse cholesterol transport
GO:0045087 innate immune response
GO:0051788 response to misfolded protein
Regulatory
For research purposes only

Applications of Clusterin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Western Blotting 0.3 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP2129 detects a band of approximately 35-37kDa in human colon, kidney and prostate cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader