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CDK5 antibody

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Western Blot Validated
Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with CDK5 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 10 second exposure. Arrow points to CDK5 (molecular weight 30 kDa).

Rabbit anti CDK5

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00451
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human CDK5 antibody recognizes cyclin-dependent kinase 5 (CDK5), also known as Tau protein kinase II catalytic subunit, cyclin-dependent-like kinase 5, protein kinase CDK5 splicing or serine/threonine-protein kinase PSSALRE. Mutations in the Cdk5 gene are a cause of lissencephaly 7, with cerebellar hypoplasia (LIS7), a condition characterized by agyria or pachygyria with disruption of cortical lamination (Magen et al. 2015).

Rabbit anti Human CDK5 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CDK5 detects a band of approximately 30 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the N terminal region of human CDK5

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q00535
Entrez Gene
CDK5
GO Terms
GO:0016020 membrane
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0043125 ErbB-3 class receptor binding
GO:0005176 ErbB-2 class receptor binding
GO:0005524 ATP binding
GO:0009790 embryo development
GO:0008283 cell proliferation
GO:0030027 lamellipodium
GO:0030424 axon
GO:0030425 dendrite
GO:0030426 growth cone
GO:0030549 acetylcholine receptor activator activity
GO:0031594 neuromuscular junction
GO:0043025 neuronal cell body
GO:0043525 positive regulation of neuron apoptosis
GO:0050321 tau-protein kinase activity
GO:0051301 cell division
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of CDK5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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