CDK5 antibody
Rabbit anti CDK5
Rabbit anti-human CDK5 antibody recognizes cyclin-dependent kinase 5, also known as protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE or tau protein kinase II catalytic subunit.
CDK5 Antibody with Control Lysate
Rabbit anti-human CDK5 antibody recognizes cyclin-dependent kinase 5, also known as protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE or tau protein kinase II catalytic subunit.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00451 | WB | 100 µl | ||||
| VPA00451KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human CDK5 antibody recognizes cyclin-dependent kinase 5 (CDK5), also known as Tau protein kinase II catalytic subunit, cyclin-dependent-like kinase 5, protein kinase CDK5 splicing or serine/threonine-protein kinase PSSALRE. Mutations in the Cdk5 gene are a cause of lissencephaly 7, with cerebellar hypoplasia (LIS7), a condition characterized by agyria or pachygyria with disruption of cortical lamination (Magen et al. 2015).
Rabbit anti Human CDK5 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Rabbit anti Human CDK5 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti CDK5 detects a band of approximately 30 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Product Form
- Purified IgG - liquid.
- Preparation
- Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
- Preparation
- 20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose. - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
2% Sucrose. - Immunogen
- Synthetic peptide directed towards the N terminal region of human CDK5
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q00535 Related reagents
- Entrez Gene
- CDK5 Related reagents
- GO Terms
- GO:0016020 membrane
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0004693 cyclin-dependent protein kinase activity
- GO:0043125 ErbB-3 class receptor binding
- GO:0005176 ErbB-2 class receptor binding
- GO:0005524 ATP binding
- GO:0009790 embryo development
- GO:0008283 cell proliferation
- GO:0030027 lamellipodium
- GO:0030424 axon
- GO:0030425 dendrite
- GO:0030426 growth cone
- GO:0030549 acetylcholine receptor activator activity
- GO:0031594 neuromuscular junction
- GO:0043025 neuronal cell body
- GO:0043525 positive regulation of neuron apoptosis
- GO:0050321 tau-protein kinase activity
- GO:0051301 cell division
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only.
Applications of CDK5 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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