CDK5 antibody

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Rabbit anti CDK5

Rabbit anti-human CDK5 antibody recognizes cyclin-dependent kinase 5, also known as protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE or tau protein kinase II catalytic subunit.

CDK5 Antibody with Control Lysate

Rabbit anti-human CDK5 antibody recognizes cyclin-dependent kinase 5, also known as protein kinase CDK5 splicing, serine/threonine-protein kinase PSSALRE or tau protein kinase II catalytic subunit.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00451 WB 100 µl
VPA00451KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human CDK5 antibody recognizes cyclin-dependent kinase 5 (CDK5), also known as Tau protein kinase II catalytic subunit, cyclin-dependent-like kinase 5, protein kinase CDK5 splicing or serine/threonine-protein kinase PSSALRE. Mutations in the Cdk5 gene are a cause of lissencephaly 7, with cerebellar hypoplasia (LIS7), a condition characterized by agyria or pachygyria with disruption of cortical lamination (Magen et al. 2015).

Rabbit anti Human CDK5 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CDK5 detects a band of approximately 30 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Product Form
Purified IgG - liquid.
Preparation
Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
Preparation
20μl Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
2% Sucrose.
Immunogen
Synthetic peptide directed towards the N terminal region of human CDK5
Instructions For Use
Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q00535 Related reagents
Entrez Gene
CDK5 Related reagents
GO Terms
GO:0016020 membrane
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004693 cyclin-dependent protein kinase activity
GO:0043125 ErbB-3 class receptor binding
GO:0005176 ErbB-2 class receptor binding
GO:0005524 ATP binding
GO:0009790 embryo development
GO:0008283 cell proliferation
GO:0030027 lamellipodium
GO:0030424 axon
GO:0030425 dendrite
GO:0030426 growth cone
GO:0030549 acetylcholine receptor activator activity
GO:0031594 neuromuscular junction
GO:0043025 neuronal cell body
GO:0043525 positive regulation of neuron apoptosis
GO:0050321 tau-protein kinase activity
GO:0051301 cell division
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only.

Applications of CDK5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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