CDK5 antibody

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Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3

Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 4

Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CDK5 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to CDK5 (molecular weight 30 kDa).

Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Western blot analysis of CDK5 knockout HEK293 and wild type HEK293 (WT) whole cell lysates probed with Rabbit anti CDK5 antibody (**VPA00451**) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000 STAR208P) and visualized on the ChemiDoc MP with a 12 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.

Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3 — Normalized signal intensity of CDK5 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Anti CDK5 Antibody (PrecisionAb Polyclonal Antibody) gallery image 4 — Western blot analysis of whole cell lysates probed with CDK5 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 10 second exposure. Arrow points to CDK5 (molecular weight 30 kDa).

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Rabbit anti CDK5

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: CDK5

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VPA00451 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human CDK5 antibody recognizes cyclin-dependent kinase 5 (CDK5), also known as Tau protein kinase II catalytic subunit, cyclin-dependent-like kinase 5, protein kinase CDK5 splicing or serine/threonine-protein kinase PSSALRE. Mutations in the Cdk5 gene are a cause of lissencephaly 7, with cerebellar hypoplasia (LIS7), a condition characterized by agyria or pachygyria with disruption of cortical lamination (Magen et al. 2015).

Rabbit anti Human CDK5 antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CDK5 detects a band of approximately 30 kDa in Jurkat cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Rabbit polyclonal antibody prepared by affinity chromatography on Protein A.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
2% Sucrose.
Immunogen: Synthetic peptide directed towards the N terminal region of human CDK5
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q00535
Entrez Gene: CDK5
GO Terms: GO:0016020 membrane; GO:0007596 blood coagulation; GO:0005829 cytosol; GO:0004693 cyclin-dependent protein kinase activity; GO:0043125 ErbB-3 class receptor binding; GO:0005176 ErbB-2 class receptor binding; GO:0005524 ATP binding; GO:0009790 embryo development; GO:0008283 cell proliferation; View More GO Terms; GO:0030027 lamellipodium; GO:0030424 axon; GO:0030425 dendrite; GO:0030426 growth cone; GO:0030549 acetylcholine receptor activator activity; GO:0031594 neuromuscular junction; GO:0043025 neuronal cell body; GO:0043525 positive regulation of neuron apoptosis; GO:0050321 tau-protein kinase activity; GO:0051301 cell division