CD274 antibody
Goat anti Human CD274 antibody (AHP2128) used for the identification of PD-L1 expression by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical positive control (original magnification × 400). a Programmed cell death protein 1 receptor (PD-1): lymph node (germinal center in follicle). b Programmed cell death protein 1 receptor ligand (PD-L1): skeletal muscle. c PD-L2: lymph node (subcapsular sinus)
From: Duchnowska R, Pęksa R, Radecka B, Mandat T, Trojanowski T, Jarosz B, Czartoryska-Arłukowicz B, Olszewski WP, Och W, Kalinka-Warzocha E, Kozłowski W, Kowalczyk A, Loi S, Biernat W, Jassem J; Polish
Brain Metastasis Consortium.
Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
Breast Cancer Res. 2016 Apr 27;18(1):43.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Human CD274 antibody (AHP2128) used for the identification of PD-L1 expression by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis (original magnification × 200). a CD4+ lymphocytes. b CD8+ lymphocytes. c CTLA4– lymphocytes. d CD68+ cells. e Glial fibrillary acid protein (GFAP) weak positive-reactive astrocytes. f GFAP moderate positive-reactive astrocytes. g Programmed cell death protein 1 receptor (PD-1)-negative expression on stromal tumor infiltrating lymphocytes (TILs) in the brain microenvironment. h PD-1-positive expression on TILs in the brain microenvironment. i PD-L1 negative expression on TILs in brain microenvironment. j Programmed cell death protein 1 receptor ligand (PD-L1)-negative expression in breast cancer brain metastases (BCBM). k PD-L1 weak positive expression in BCBM. l PD-L1 moderate positive expression in BCBM. m PD-L2 negative expression in TILs in the brain microenvironment. n PD-L2 negative expression in BCBM. o PD-L2 weak positive expression in BCBM. p PD-L2 moderate positive expression in BCBM.
From: Duchnowska R, Pęksa R, Radecka B, Mandat T, Trojanowski T, Jarosz B, Czartoryska-Arłukowicz B, Olszewski WP, Och W, Kalinka-Warzocha E, Kozłowski W, Kowalczyk A, Loi S, Biernat W, Jassem J;
Polish Brain Metastasis Consortium.
Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
Breast Cancer Res. 2016 Apr 27;18(1):43.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
WB P ResetGoat anti Human CD274 (C-Terminal)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CD274
- Region
- (C-TERMINAL)
Antibody Quality and Performance Guarantee
| Goat anti Human CD274 antibody recognizes an epitope within the C-terminal (CT) region of human CD274, otherwise known as PD-L1, a cell surface glycoprotein and member of the B7 family of co-stimulatory molecules, constitutively expressed on macrophages and dendritic cells, and inducible on activated T-cells, B-cells, endothelial cells and epithelial cells, in response to interferons. CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human CD274 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence CKKQSDTHLEET from the C-Terminal region of CD274 (NP_054862.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/8000 | |
| Immunohistology - Paraffin 1 | |
4.0 | 6.0ug/ml |
| Western Blotting | |
0.3 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human skeletal muscle
- Western Blotting
- AHP2128 detects a band of approximately 35kDa in human heart cell lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):FITC | ||||||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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| Description | Rabbit anti Goat IgG (Fc):HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
References for CD274 antibody
-
Duchnowska, R. et al. (2016) Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
Breast Cancer Res. 18 (1): 43.
- Synonyms
- PD-L1
- RRID
- AB_2073434
- UniProt
- Q9NZQ7
- Entrez Gene
- CD274
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008283 cell proliferation
- GO:0012505 endomembrane system
- GO:0031295 T cell costimulation
AHP2128
148067
151949
158229
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