CD274 antibody
Goat anti Human CD274 antibody (AHP2128) used for the identification of PD-L1 expression by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical positive control (original magnification × 400). a Programmed cell death protein 1 receptor (PD-1): lymph node (germinal center in follicle). b Programmed cell death protein 1 receptor ligand (PD-L1): skeletal muscle. c PD-L2: lymph node (subcapsular sinus)
From: Duchnowska R, Pęksa R, Radecka B, Mandat T, Trojanowski T, Jarosz B, Czartoryska-Arłukowicz B, Olszewski WP, Och W, Kalinka-Warzocha E, Kozłowski W, Kowalczyk A, Loi S, Biernat W, Jassem J; Polish Brain Metastasis Consortium.
Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
Breast Cancer Res. 2016 Apr 27;18(1):43.
Goat anti Human CD274 antibody (AHP2128) used for the identification of PD-L1 expression by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis (original magnification × 200). a CD4+ lymphocytes. b CD8+ lymphocytes. c CTLA4– lymphocytes. d CD68+ cells. e Glial fibrillary acid protein (GFAP) weak positive-reactive astrocytes. f GFAP moderate positive-reactive astrocytes. g Programmed cell death protein 1 receptor (PD-1)-negative expression on stromal tumor infiltrating lymphocytes (TILs) in the brain microenvironment. h PD-1-positive expression on TILs in the brain microenvironment. i PD-L1 negative expression on TILs in brain microenvironment. j Programmed cell death protein 1 receptor ligand (PD-L1)-negative expression in breast cancer brain metastases (BCBM). k PD-L1 weak positive expression in BCBM. l PD-L1 moderate positive expression in BCBM. m PD-L2 negative expression in TILs in the brain microenvironment. n PD-L2 negative expression in BCBM. o PD-L2 weak positive expression in BCBM. p PD-L2 moderate positive expression in BCBM.
From: Duchnowska R, Pęksa R, Radecka B, Mandat T, Trojanowski T, Jarosz B, Czartoryska-Arłukowicz B, Olszewski WP, Och W, Kalinka-Warzocha E, Kozłowski W, Kowalczyk A, Loi S, Biernat W, Jassem J; Polish Brain Metastasis Consortium.
Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
Breast Cancer Res. 2016 Apr 27;18(1):43.
Goat anti Human CD274 antibody recognizes an epitope within the C-terminal (CT) region of human CD274, otherwise known as PD-L1, a cell surface glycoprotein and member of the B7 family of co-stimulatory molecules, constitutively expressed on macrophages and dendritic cells, and inducible on activated T-cells, B-cells, endothelial cells and epithelial cells, in response to interferons.
CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1.
CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human CD274 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence CKKQSDTHLEET from the C-Terminal region of CD274 (NP_054862.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- Q9NZQ7 Related reagents
- Entrez Gene
- CD274 Related reagents
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008283 cell proliferation
- GO:0012505 endomembrane system
- GO:0031295 T cell costimulation
- Regulatory
- For research purposes only
Applications of CD274 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/8000 | ||
| Immunohistology - Paraffin 1 | 4.0 | 6.0ug/ml | |
| Western Blotting | 0.3 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human skeletal muscle
- Western Blotting
- AHP2128 detects a band of approximately 35kDa in human heart cell lysates.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P |
Product Specific References
References for CD274 antibody
-
Duchnowska, R. et al. (2016) Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
Breast Cancer Res. 18 (1): 43.
