CD274 antibody




CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human CD274 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Synthetic peptide sequence CKKQSDTHLEET from the C-Terminal region of CD274 (NP_054862.1).
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9NZQ7
- Entrez Gene
- CD274
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008283 cell proliferation
- GO:0012505 endomembrane system
- GO:0031295 T cell costimulation
- Regulatory
- For research purposes only
Applications of CD274 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/8000 | ||
Immunohistology - Paraffin 1 | 4.0 | 6.0ug/ml | |
Western Blotting | 0.3 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Histology Positive Control Tissue
- Human skeletal muscle
- Western Blotting
- AHP2128 detects a band of approximately 35kDa in human heart cell lysates.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
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Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml |
Product Specific References
References for CD274 antibody
-
Duchnowska, R. et al. (2016) Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
Breast Cancer Res. 18 (1): 43.
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