CD274 antibody

Goat anti Human CD274 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD274
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP2128
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human CD274 antibody recognizes an epitope within the C-terminal (CT) region of human CD274, otherwise known as PD-L1, a cell surface glycoprotein and member of the B7 family of co-stimulatory molecules, constitutively expressed on macrophages and dendritic cells, and inducible on activated T-cells, B-cells, endothelial cells and epithelial cells, in response to interferons.

CD274 acts as both a co-stimulatory and co-inhibitory molecule, co-stimulating early T cell priming, differentiation and IL-10 production, and inhibiting T cell proliferation and TCR-mediated activation of IL-2 production, through interaction with the receptor CD279/PD-1.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human CD274 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide sequence CKKQSDTHLEET from the C-Terminal region of CD274 (NP_054862.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000
Immunohistology - Paraffin 1 4.0 6.0ug/ml
Western Blotting 0.3 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human skeletal muscle
Western Blotting
AHP2128 detects a band of approximately 35kDa in human heart cell lysates.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader
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loader
Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0

References for CD274 antibody

  1. Duchnowska, R. et al. (2016) Immune response in breast cancer brain metastases and their microenvironment: the role of the PD-1/PD-L axis.
    Breast Cancer Res. 18 (1): 43.

Synonyms
PD-L1
RRID
AB_2073434
UniProt
Q9NZQ7
Entrez Gene
CD274
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0006955 immune response
GO:0007166 cell surface receptor linked signaling pathway
GO:0008283 cell proliferation
GO:0012505 endomembrane system
GO:0031295 T cell costimulation
View more products with CD274 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products