CD274 antibody
Rabbit antiHuman CD274 antibody (AHP1703) used for the evaluation of PD-L1 expression on HCT-116 and HEK-293 cells by immunofluorescence and flow cytometry.
Image caption:
Immunofluorescent staining of HCT-116 and HEK-293 cells for PD-L1 (red), anti-CSV 84-1 (green), and nuclei (blue).
(A) Stains as a group of cells. (B) Stains of single cell. (C) HEK-293 cells stained for PD-L1 (red) and nuclei (blue). (D) HEK-293 cells transfected with PD-L1 stained for PD-L1 (red) and nuclei (blue). The images were taken using confocal microscopy. Scale bar, 10 μm. (E) Flow cytometric evaluation of intracellular PD-L1 expression vector control-transfected (left panel) and PD-L1–transfected (right panel) HEK-293 cells.
From: Satelli A, Batth IS, Brownlee Z, Rojas C, Meng QH, Kopetz S, Li S.
Potential role of nuclear PD-L1 expression in cell-surface vimentin positive circulating tumor cells as a prognostic marker in cancer patients.
Sci Rep. 2016 Jul 1;6:28910.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit antiHuman CD274 antibody (AHP1703) used for the evaluation of PD-L1 expression on circulating tumor cells from cancer patients by immunofluorescence.
Image caption:
PD-L1 expression in CTCs isolated from prostate cancer, breast cancer, colon cancer, and osteosarcoma patients.
Cells were stained for CSV (green) using the 84-1 antibody, PD-L1 (red), and SYTOX green (pseudo blue). The images were taken using confocal microscopy. Scale bar, 10 μm.
From: Satelli A, Batth IS, Brownlee Z, Rojas C, Meng QH, Kopetz S, Li S.
Potential role of nuclear PD-L1 expression in cell-surface vimentin positive circulating tumor cells as a prognostic marker in cancer patients.
Sci Rep. 2016 Jul 1;6:28910.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Human CD274
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- CD274
Antibody Quality and Performance Guarantee
| Rabbit anti Human CD274 antibody detects CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein, a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naïve T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to co-stimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Human CD274 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to a 17 amino acid sequence from near the centre of Human CD274.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Paraffin 1 | |
2.5ug/ml | |
| Western Blotting | |
0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human heart tissue
- Western Blotting
- AHP1703 detects a band of approximately 32kDa in Raji cell lysate.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for CD274 antibody
-
Satelli, A. et al. (2016) Potential role of nuclear PD-L1 expression in cell-surface vimentin positive circulating tumor cells as a prognostic marker in cancer patients.
Sci Rep. 6: 28910.
Further Reading
-
Lagier, A.J. & Pober, J.S. (2006) Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1).
Hum Immunol. 67 (8): 568-78. -
Holling, T.M. et al. (2004) Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men.
Hum Immunol. 65 (4): 282-90. -
Ishida, Y. et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.
EMBO J. 11 (11): 3887-95.
- Synonyms
- PD-L1
- RRID
- AB_2073436
- UniProt
- Q9NZQ7
- Entrez Gene
- CD274
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008283 cell proliferation
- GO:0012505 endomembrane system
- GO:0031295 T cell costimulation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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