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CD14 antibody

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Anti Human CD14 Antibody gallery image 1
Western blotting analysis of human PBL lysate (35μg protein in RIPA buffer) with (Lane B) and without (Lane A) blocking with the immunizing peptide using Goat anti Human CD14 antibody (AHP1059) at 1μgml-1. Primary incubation was 1 hour. Detected by chemiluminescence.

Goat anti Human CD14

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1059
Datasheet
E P* WB
SDS
0.1 mg

Goat anti Human CD14 antibody recognizes human CD14, a 55 kDa receptor for endotoxin and other bacterial cell wall components.Goat anti Human CD14 antibody detects an epitope within the internal region of CD14. CD14 acts as a multiligand pattern recognition receptor, inducing specific coassembly of additional receptors, including MD-2 and TLR4 as part of the lipopolysaccharide (LPS) receptor.

The LPS receptor is expressed strongly on the surface of monocytes and weakly on the surface of granulocytes, and is also expressed by most tissue macrophages. Activation of the LPS receptor leads to NF-kappa-B activation, cytokine secretion and the inflammatory response. CD14 also up-regulates cell surface molecules, including adhesion molecules, and has been shown to bind apoptotic cells

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to CD14 were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using the immunizing peptide, reactivity with the carrier protein was removed during purification.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence C-KRVDADADPRQYAD corresponding to amino acid residues 71-84 of the human CD14 precursor conjugated to a carrier protein.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P08571
Entrez Gene
CD14
GO Terms
GO:0005886 plasma membrane
GO:0001530 lipopolysaccharide binding
GO:0001847 opsonin receptor activity
GO:0006915 apoptosis
GO:0006909 phagocytosis
GO:0006954 inflammatory response
GO:0008063 Toll signaling pathway
GO:0031225 anchored to membrane
GO:0016019 peptidoglycan receptor activity
GO:0032760 positive regulation of tumor necrosis factor production
GO:0045087 innate immune response
GO:0070891 lipoteichoic acid binding
GO:0071222 cellular response to lipopolysaccharide
GO:0071223 cellular response to lipoteichoic acid
Regulatory
For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/128,000
Immunohistology - Paraffin 1 4 6 ug/ml
Western Blotting 1 3 ug/ml
  1. 1Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Citrate buffer, pH6.0, is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human Spleen
Western Blotting
AHP1059 detects a band of approximately 55kD in human lung, lymph node and tonsil lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Product Specific References

References for CD14 antibody

  1. Triantafilou, M. & Triantafilou, K. (2002) Lipopolysaccharide recognition: CD14, TLRs and the LPS-activation cluster.
    Trends Immunol. 23 (6): 301-4.
  2. Miyake, K. (2003) Innate recognition of lipopolysaccharide by CD14 and toll-like receptor 4-MD-2: unique roles for MD-2.
    Int Immunopharmacol. 3 (1): 119-28.

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